Skip to content
Research Article Open access CC BY 4.0

Polymorphisms of Seed Storage Proteins in Olea europaea L. Cultivars

Rodolfo Bernardi, Raffaella Petruccelli, Giorgio Bartolini, Mauro Durante

Asian Research Journal of Agriculture · pp. 1–6 · Published 24 May 2017

10.9734/ARJA/2017/33755

Abstract

The use of Olea europaea globulins as genetic markers for the identification of the cultivars was perfomed in the laboratory of Genetics of Department of Agriculture, Food and Environment, University of Pisa. The major component of Olea europaea L. seed storage proteins is represented by globulins. These fractions were characterised by electrophoresis and compared with the proteins extracted from protein bodies. The biochemical analysis of the olive seed globulins was carried out in sixteen different cultivars coming from several geographical areas of Italy. The electrophoretic patterns in polyacrylamide gels electrophoresis in denaturing conditions (SDS-PAGE) evidenced both qualitative and quantitative differences. It was possible to identify all the cultivars by             their electrophoretic spectra. Number and position of the electrophoretic bands allowed the construction of a similarity matrix and of a dendrogram that allowed the separation into groups, according to their phylogenetic relationships. The several clusters seem to be related with agronomic traits such as fruit size or oil production; no relationships were found with the geographical cultivation areas.

Olea europaea L. protein bodies seed storage proteins gel electrophoresis genetic distances cultivar identification

References (12)

  1. 1 Mathematical model for studying genetic variation in terms of restriction endonucleases. [DOI]
  2. 2 Allozyme diversity in wild Phaseolus vulgaris: further evidence for two major centers of genetic diversity [DOI]
  3. 3 Allozyme variation of oleaster populations (wild olive tree) (Olea europaea L.) in the Mediterranean Basin [DOI]
  4. 4 Seed protein electrophoresis in taxonomic and evolutionary studies [DOI]
  5. 5 History of olive cultivars based on their genetic distances [DOI]
  6. 6 Genetic variation in the subunits of globulin-1 storage protein of French bean [DOI]
  7. 7 Biochemical Characterization and Cellular Localization of 11S Type Storage Proteins in Olive (Olea europaea L.) Seeds [DOI]
  8. 8 Separation of olive proteins by capillary gel electrophoresis [DOI]
  9. 9 Leaf Allozyme Variation in Cultivated and Wild Olive Trees (Olea europaea L.) [DOI]
  10. 10 Characterization of Helianthus annuus L. storage proteins [DOI]
  11. 11 COMPARTMENTALIZATION IN THE COTYLEDONARY CELLS OF PHASEOLUS VULGARIS L. SEEDS: A DIFFERENTIAL SEDIMENTATION STUDY [DOI]
  12. 12 Phaseolus coccineus L. Storage Proteins. Extraction and Characterization [DOI]

Cited by 1

1 citation reported by external sources — individual citing-article records aren't available to list yet.

Related research

Article metrics

Real usage data collected on this platform.

23

Page views

0

PDF downloads

3

Outbound clicks

1

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.