Skip to content
Research Article Open access CC BY 3.0

Pharmacognostic and HPTLC Fingerprint Profile of the Root of Aristolochia indica Linn. and Quantification of the Marker Compound

M. V. Sudhakaran

European Journal of Medicinal Plants · pp. 1113–1124 · Published 16 Jun 2014

10.9734/EJMP/2014/9570

Abstract

Introduction: Root of Aristolochia indica Linn. has long been used as an oxytoxic agent to aid women in child birth and as abortifacient in Indian folk medicine. It is also one of the ingredients in some traditional Ayurveda medicinal preparations. Aims: The present work has been designed to delineate the pharmacognostic profile of the root of Aristolochia indica Linn and the High-performance thin-layer chromatographic (HPTLC) identification of the active compound and its quantitative estimation in the herbal sample. Materials and Methods: Macroscopic, microscopic evaluation, powder analysis, fluorescence standards of the root of Aristolochia indica Linn and its HPTLC fingerprint profile. Results:  Pharmacognostic profile of the root investigated revealed the transverse section possessing somewhat circular outline with tissue organization as outer thin walled cork layers, narrow cortex, and inner cortical cells with groups of stone cells. Secondary xylem tissues were fissured to form narrow strips, wide medullary rays with greater quantities of parenchyma, ray cells with rich deposition of starch. Vessels were solitary and occluded with tyloses and starch grains with 'Maltese cross' were the characteristic features of the taxon. HPTLC method was developed for the estimation of the marker constituent, Aristolochic Acid I (AAI) in dried root sample. Chloroform: Methanol (6:2v/v), was used as mobile phase to separate the analyte. The Rf value for Aristolochic Acid I (C17H11NO7) was found to be 0.53. Calibration plot was established showing the dependence of response on the amount chromatographed. Linearity was found to be in the concentration range of 100 to 500ng/spot for AAI with the correlation coefficient value r=0.998. The result showed that the content of marker compound (AAI) in dried root of Aristochia india Linn was 0.082%. Conclusions: The results of the present study suggest that, the documented morphological descriptors, delineated anatomical markers and developed HPTLC methods are complementary characteristics, which could be effectively used for the identification and authentication of the root of Aristolochia indica Linn.  

Aristolochia indica Linn microscopic determination HPTLC finger print active compound

References (13)

  1. 1 Glossary of Indian Medicinal Plants
  2. 2 General guidelines for methodologies on research and evaluation of traditional medicine
  3. 3 Urothelial Carcinoma Associated with the Use of a Chinese Herb (Aristolochia fangchi) [DOI]
  4. 4 Flora of the Presidency of Madras [DOI]
  5. 5 UROTHELIAL CARCINOMA ASSOCIATED WITH THE USE OF A CHINESE HERB ( ARISTOLOCHIA FANGCHI )
  6. 6 Misuse of Herbal Remedies: The Case of an Outbreak of Terminal Renal Failure in Belgium (Chinese Herbs Nephropathy) [DOI]
  7. 7 Urothelial malignant disease and Chinese herbal nephropathy [DOI]
  8. 8 Flora of the Presidency of Madras [DOI]
  9. 9 Botanical pharmacognosy of Andrographis paniculata (Burm. F.) Wall. Ex. Nees [DOI]
  10. 10 WOOD ANATOMY AND RELATIONSHIPS OF LACTORIDACEAE [DOI]
  11. 11 An N-glycoside and steroids from Aristolochia indica [DOI]
  12. 12 Wood Anatomy and Relationships of Lactoridaceae [DOI]
  13. 13 Histo-morphological, fluorescent and powder microscopic characterization of Cissampelos pareira Linn. [DOI]

Cited by 9

Genetic diversity assessment and biotechnological aspects in Aristolochia spp.

Shreya Nath, Nilanjana Ghosh, Tauhid Alam Ansari · Applied Microbiology and Biotechnology · 2022

Botanical Pharmacognosy of Holostemma ada-kodien Schult

Sudhakaran Madathilparambil Vasu · Pharmacognosy Journal · 2017

Polyamine elicited aristolochic acid production in in vitro clonally fidel Aristolochia indica L.: An ISSR and RAPD markers and HPTLC based study

Abhijit Dey, Potsangbam Nongdam, Samapika Nandy · South African Journal of Botany · 2021

Showing 3 of 9 known citations — external sources report more than can currently be individually listed.

Article metrics

Real usage data collected on this platform.

11

Page views

0

PDF downloads

0

Outbound clicks

9

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.