Skip to content
Research Article Open access CC BY 4.0

Oxidant–Antioxidant Status and Renal Function in Wistar Rats after Administration of Vernonia amygdalina Fractions in Streptozotocin Induced Diabetes Mellitus

Anthony E. Ojieh, Emmanuel A. Ugorji, Micheal E. Aisuodionoe, Johnson Uyovwiesevwa, Eghworo Ovocity, Ewhre O. Lawrence, Ikenna P. Njoku, Christopher U. Onyekpe, Daubry M. E. Tarela

Journal of Advances in Medical and Pharmaceutical Sciences · pp. 1–12 · Published 9 May 2016

10.9734/JAMPS/2016/25404

Abstract

Diabetes mellitus is a group of metabolic diseases characterized by sustained hyperglycemia resulting from defects in insulin secretion, insulin action, or both. It accounts for about 3% of all deaths globally and affects all glucose utilizing tissue leading to severe complications. One third of diabetes patients eventually develops renal complications and is the major cause of end stage renal disease the pathogenesis of which has been associated to oxidative stress. An alternative approach in the management of diabetes has focused on the role of herbs. In this study, Vernonia amygdalina a tropical savanna herb was studied for its potent antioxidant property and its ameliorative effect on diabetic renal complication. 40 adult male wistar rats were divided into eight groups (n=5) A-H. Fresh Vernonia amygdalina leaves were extracted and fractionated using four solvents of varying polarity. The extracts were further concentrated and reconstituted and was administered to the animals. Group A were used as control, groups B-H were induced diabetes with a single intravenous injection of 60 mg/kg B.W. streptozotocin. Group B were induced with diabetes but left untreated, group C received metformin (50 mg/kg B.W.) while groups D-H received 300 mg/kg B.W of crude Vernonia and the respective fractions of the plants. Laboratory analysis at the end of the analysis showed that the plant crude and fraction reduced the level of fasting blood glucose between 30– 80%, increased serum catalase and superoxide dismutase level while decreasing malondialdehyde level. The plant caused significant reduction in serum creatinine level compared to the untreated diabetic groups and the control groups. The study shows that Vernonia amygdalina reduces oxidative stress by causing a balance in the oxidant–antioxidant level as well restores deranged renal function seen in diabetes mellitus.  

Diabetes oxidative stress renal function streptozotocin Vernonia amygdalina antioxidants

References (20)

  1. 1 Biochemistry and molecular cell biology of diabetic complications [DOI]
  2. 2 The Role of Superoxide Anion in the Autoxidation of Epinephrine and a Simple Assay for Superoxide Dismutase [DOI]
  3. 3 Chemistry and biochemistry of 4-hydroxynonenal, malonaldehyde and related aldehydes [DOI]
  4. 4 The role of oxidative stress in the onset and progression of diabetes and its complications: asummary of a Congress Series sponsored byUNESCO-MCBN, the American Diabetes Association and the German Diabetes Society [DOI]
  5. 5 Phytochemical Screening and Antioxidant Activities of Some Selected Medicinal Plants Used for Malaria Therapy in Southwestern Nigeria [DOI]
  6. 6 PATHOGENESIS OF TYPE 2 DIABETES: METABOLIC AND MOLECULAR IMPLICATIONS FOR IDENTIFYING DIABETES GENES
  7. 7 Renal function in diabetic nephropathy [DOI]
  8. 8 Effect of aloes on blood glucose levels in normal and alloxan diabetic mice [DOI]
  9. 9 Efficacy of aqueous leaf extract of vernonia amygdalina on plasma lipoprotein and oxidative status in diabetic rat models.
  10. 10 Hepatoprotective and Antioxidant Activities of Vernonia amygdalina on Acetaminophen-Induced Hepatic Damage in Mice [DOI]
  11. 11 The Expression of Different Superoxide Dismutase Forms is Cell-type Dependent in Olive ( Olea europaea L.) Leaves [DOI]
  12. 12 Effect of Vernonia amygdalina Del Leaf on Kidney Function of Diabetic Rats [DOI]
  13. 13 Hypoglycemic indices of Vernonia amygdalina on postprandial blood glucose concentration of healthy humans [DOI]
  14. 14 Amelioration of carbon tetrachloride-induced hepatotoxicity by terpenoid extract from leaves of Vernonia amydgalina.
  15. 15 Madagascar periwinkle (Catharanthus roseus) enhances kidney and liver functions in Wistar rats
  16. 16 Protective Effect of Some Tropical Vegetables Against CCl 4 -Induced Hepatic Damage [DOI]
  17. 17 Aqueous Extract of Vernonia amygdalina Protects Against Alcohol-Induced Hepatotoxicity in Wistar Rats
  18. 18 Evaluation of Albuminuria, Total Plasma Proteins, and Serum Albumin in Diabetics
  19. 19 Effect of Activity Directed Fractions of Vernonia amygdalina on Total Body Weight and Blood Glucose Levels of Diabetic Wistar Albino Rats
  20. 20 Hypertension, Angiotensin II, and Oxidative Stress [DOI]

Cited by 2

Effects of Vernonia Amygdalina Leaf Extract on Total Antioxidant Capacity and Malondialdehyde Levels in Acetaminophen-Induced Toxicity in Mice

Mweenda Sitanimezi, Ezeala Christian Chinyere, Prashar Lavina · 2017

The effects of Vernonia amygdalina(Asteraceae) Delile methanolic leaf extract on markers of oxidative stress in mice treated with high doses of acetaminophen

Sitanimezi Mweenda · 2017

Related research

Article metrics

Real usage data collected on this platform.

73

Page views

0

PDF downloads

31

Outbound clicks

2

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.