Skip to content
Research Article Open access CC BY 3.0

Optimization of Culture Conditions for the Production of Extracellular Cellulases via Solid State Fermentation

Dina H. I. El-Ghonemy, Thanaa H. Ali, Maysa E. Moharam

Microbiology Research Journal International · pp. 698–714 · Published 12 Mar 2014

10.9734/BMRJ/2014/8100

Abstract

Aim: The aims of the present study were to screen different filamentous fungi for extracellular cellulases production and to optimize solid-state fermentation medium and culture conditions to enhance cellulases production. Study Design: Using agro-industrial waste as raw material for the production of cellulases by a hyper cellulase producing fungus and evaluating the influence of various parameters to design a suitable SSF process for cellulase production. Place and Duration of Study: Department of Microbial Chemistry, Genetic Engineering and Biotechnology Division, National Research Centre (NRC), Cairo, Egypt, between January 2013 and October 2013. Methodology: Different filamentous fungi were grown and maintained on potato dextrose agar slants at 28ºC for 7 days. The spores were washed down by distilled water. Then, 2.0 ml aliquots were used to inoculate 250 ml Erlenmeyer flasks, containing rice straw as the only carbon source. The inoculated flasks were incubated for 5 days at 28ºC. The enzymes were extracted by mixing homogenously the fermented substrate with 50 ml citrate phosphate buffer (0.1 M, pH 5.0) and agitated (150 rpm) for 1 hr. Pooled extracts were centrifuged at 5000 rpm for 15min and the clear supernatant was used as a source of extracellular enzyme. Results: Aspergillus oryzae NRRL 3484 exhibited relatively higher cellulases production. The optimum incubation period, temperature, and initial moisture level were reported on the 7th day, at 28°C, and 70%, respectively. Peptone proved to be the suitable nitrogen source followed by yeast extract, while pH 5.0 was ideal for cellulases production. Conclusion: Using ligninolytic fungi, including their enzymes, may be one potential alternative to provide a more practical and environmental-friendly approach for enhancing the nutritive value of rice straw. Moreover, the application of ligninolytic fungi or their enzymes combined with chemical pre-treatments to rice straw may be an alternative way to shorten the period of the incubation times and (or) decrease the amount of chemicals, effecting some synergy.

Aspergillus oryzae NRRL 3484 extracellular cellulases solid state fermentationq

References (61)

  1. 1 PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT [DOI]
  2. 2 Recent advances in solid-state fermentation [DOI]
  3. 3 Application of microbial α-amylase in industry - A review [DOI]
  4. 4 Inhibition of cellulase, xylanase and β-glucosidase activities by softwood lignin preparations [DOI]
  5. 5 Engineering aspects of solid state fermentation [DOI]
  6. 6 Production of cellulases and hemicellulases by Aspergillus niger KK2 from lignocellulosic biomass [DOI]
  7. 7 Production of fungal xylanases [DOI]
  8. 8 Utilization of Rice Straw and Different Treatments to Improve Its Feed Value for Ruminants: A Review [DOI]
  9. 9 Production and characterization of cellulolytic enzymes from the thermoacidophilic fungal Aspergillus terreus M11 under solid-state cultivation of corn stover [DOI]
  10. 10 Pretreatment by microwave/alkali of rice straw and its enzymic hydrolysis [DOI]
  11. 11 Effect of different growth parameters on endoglucanase enzyme activity by bacteria isolated from coir retting effluents of estuarine environment [DOI]
  12. 12 Production of bacterial cellulases by solid state bioprocessing of banana wastes [DOI]
  13. 13 Production of cellulase by Trichoderma reesei from dairy manure [DOI]
  14. 14 Production of multiple xylanolytic and cellulolytic enzymes by thermophilic fungus Myceliophthora sp. IMI 387099 [DOI]
  15. 15 Direct microbial conversion of wheat straw into lipid by a cellulolytic fungus of Aspergillus oryzae A-4 in solid-state fermentation [DOI]
  16. 16 Biological pretreatment of sugar cane bagasse for the production of cellulases and xylanases by Penicillium echinulatum [DOI]
  17. 17 Induction, production, repression, and de-repression of exoglucanase synthesis in Aspergillus niger [DOI]
  18. 18 Solid-State Fermentation for Production of Phytase by Rhizopus oligosporus [DOI]
  19. 19 Enhanced cellulase production of the Trichoderma viride mutated by microwave and ultraviolet [DOI]
  20. 20 Xylanase Production by Penicillium canescens 10-10c in Solid-State Fermentation [DOI]
  21. 21 Cellulolytic enzymes on lignocellulosic substrates in solid state fermentation by Aspergillus niger [DOI]
  22. 22 Cellulase Production by Aspergillus fumigatus Grown on Mixed Substrate of Rice Straw and Wheat Bran [DOI]
  23. 23 Improvement of Fungal Cellulase Production by Mutation and Optimization of Solid State Fermentation [DOI]
  24. 24 Purification and characterization of ß-glucosidase from Melanocarpus sp. MTCC 3922 [DOI]
  25. 25 Purification and characterisation of an intracellular enzyme with β-glucosidase and β-galactosidase activity from the thermophilic fungus Talaromyces thermophilus CBS 236.58 [DOI]
  26. 26 True cellulase production by Clostridium thermocellum grown on different carbon sources [DOI]
  27. 27 Cellulolytic Enzymes ProductionviaSolid-State Fermentation: Effect of Pretreatment Methods on Physicochemical Characteristics of Substrate [DOI]
  28. 28 Regulation of Expression of Cellulosomal Cellulase and Hemicellulase Genes in Clostridium cellulovorans [DOI]
  29. 29 Production Of Cellulases By Aspergillus Heteromorphus From Wheat Straw Under Submerged Fermentation [DOI]
  30. 30 Cellulase production by Trichoderma sp. on apple pomace under solid state fermentation
  31. 31 Submerged cultivation of scytalidium thermophilum on complex lignocellulosic biomass for endoglucanase production [DOI]
  32. 32 A New Look at Xylanases: An Overview of Purification Strategies [DOI]
  33. 33 Potential of solid-state fermentation enzymes of Aspergillus oryzae in biobleaching of paper pulp [DOI]
  34. 34 Some observations on rice straw with emphasis on updates of its management.
  35. 35 Efficiency of rice straw lignocelluloses degradability by Aspergillus terreus ATCC 74135 in solid state fermentation
  36. 36 The use of lignocellulosic wastes for production of cellulase by Trichoderma reesei [DOI]
  37. 37 Feeding of urea-ammonia treated rice straw: a compilation of miscellaneous reports produced by the straw utilization project (Sri Lanka.
  38. 38 Fungus β-glycosidases: immobilization and use in alkyl-β-glycoside synthesis [DOI]
  39. 39 Cellulase Production by Penicillium fellutanum Isolated from Coastal Mangrove Rhizosphere Soil [DOI]
  40. 40 Air pressure pulsation solid state fermentation of feruloyl esterase by Aspergillus niger [DOI]
  41. 41 Production of a novel raw-starch-digesting glucoamylase by Penicillium sp. X-1 under solid state fermentation and its use in direct hydrolysis of raw starch [DOI]
  42. 42 Degradation of Agro-Waste by Cellulase from Aspergillus candidus [DOI]
  43. 43 Production of Cellulolytic Enzymes by Aspergillus phoenicis in Grape Waste using Response Surface Methodology [DOI]
  44. 44 Production of cellulolytic enzymes by fungi [DOI]
  45. 45 Successive cultivation of selected cellulolytic fungi on rice straw and wheat bran for economic production of cellulases and D-xylanase [DOI]
  46. 46 Celluase Production by Aspergillus niger Isolated from Coastal Mangrove Debris [DOI]
  47. 47 The distribution characteristics of bacterial β-glucosidase activity in Taiwan strait [DOI]
  48. 48 Purification and characterization of the pectin lyase and protease produced byPenicillium velutinum grown onEichhornia crassipes under solid state fermentation [DOI]
  49. 49 Solid‐state cultivation of Chaetomium cellulolyticum on alkali‐pretreated sawdust [DOI]
  50. 50 Growth of Azospirillum irakense KBC1 on the Aryl β-Glucoside Salicin Requires either salA or salB [DOI]
  51. 51 Preparation of Buffers for Use in Enzyme Studies [DOI]
  52. 52 Engineering Aspects of Solid‐State Fermentation [DOI]
  53. 53 Production of cellulase by Aspergillus niger under submerged and solid state fermentation using coir waste as a substrate [DOI]
  54. 54 Enzyme deactivation during cellulose hydrolysis [DOI]
  55. 55 Trichoderma aureoviride 7-121, a mutant with enhanced production of lytic enzymes: its potential use in waste cellulose degradation and/or biocontrol [DOI]
  56. 56 Studies on untreated and urea-treated rice straw from three cultivation seasons: 1. Physical and chemical measurements in straw and straw fractions [DOI]
  57. 57 Cellulase Production by Bacillus subtilis isolated from Cow Dung
  58. 58 Enhanced Production of Cellulase(S) By Aspergillus spp. Isolated From Agriculture Wastes by Solid State Fermentation
  59. 59 Comparative study of two purified inulinases from thermophile Thielavia Terrestris NRRL 8126 and mesophile Aspergillus Foetidus NRRL 337 grown on Cichorium Intybus l [DOI]
  60. 60 Purification and some kinetic properties of b-glucosidase from Aspergillus terreus NRRL 265 [DOI]
  61. 61 SCREENING STUDIES ON THE FORMATION OF β-GLUCOSIDASE FROM SOME FILAMENTOUS FUNGI

Cited by 17

Designing microbial cellulases using genetic engineering approach: A promising strategy towards zero-waste cellulosic biorefinery

Komal Agrawal, Lakshana G. Nair, V. Chaturvedi · Biocatalysis and Agricultural Biotechnology · 2023

Cost‐effective cellulase production, improvement strategies, and future challenges

Nikita Bhati, Shreya, A. Sharma · Journal of food process engineering · 2020

Solid state fermentation for production of microbial cellulases: Recent advances and improvement strategies.

S. Behera, R. Ray · International Journal of Biological Macromolecules · 2016

Genetic Engineering Applications to Improve Cellulase Production and Efficiency: Part II

P. Misra, P. Shukla, K. Rao · New and Future Developments in Microbial Biotechnology and Bioengineering · 2019

Solid-state fermentation for the production of microbial cellulases

Sudhanshu S. Behera, Ankush Kerketta, Ramesh C. Ray · Biotechnology of Microbial Enzymes · 2023

Showing 10 of 17 known citations — external sources report more than can currently be individually listed.

Article metrics

Real usage data collected on this platform.

41

Page views

0

PDF downloads

9

Outbound clicks

17

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.