Ameliorative Potentials and Tissue-Protective Effects of Methanolic Extract of Persea americana Seeds on Renal Function in Alloxan-Induced Diabetic Wistar Rats

Abstract

Introduction: Diabetic nephropathy is a leading cause of end-stage renal failure and chronic kidney disease throughout the globe. Despite the fact that much research has been conducted in both basic science and clinical therapeutics to better understanding of the biology of diabetic nephropathy and increase the number of potential medicines available, this research aimed to assess the ameliorative potentials and tissue-protective effects of Persea Americana Seeds aqueous extract on renal function in Alloxan-Induced Diabetic Wistar Rats. Materials and Methods: Methanol was used to remove the seeds of Persea Americana, which were then refrigerated until use. Thirty mature male Wistar rats were split into six groups of five. Group 2 to 6 rats were given a single intraperitoneal injection of alloxan (150 mg/kg) to cause diabetes. Normal and diabetic control groups are represented by groups 1 and 2, accordingly. Persea Americana Seeds extract was given to groups 3, 4, and 5 at doses of 120, 240, and 480 mg/kg/day, respectively, while metformin was given to group 6. After 14 days of treatment, the animals were sacrificed, blood samples were taken, and serum was centrifuged for biochemical analysis. The kidney were excised for histomorphological and biochemical analysis. The data were analyzed using the SPSS package, version 23, and the findings were expressed as Mean SEM. Results: Significant decreases in blood glucose, urea, creatinine, and MDA levels were observed in all diabetic groups treated with the plant extract, but there was no significant change in potassium and bicarbonate ions. However, there was a significant increase in Na+ and Cl-, Catalase, and SOD activities in all diabetic treated rats. Following therapy, the kidney tissues of infected animals were found to be recovered from the toxic effects of alloxan induction. Conclusion: Persea Americana seed has anti-diabetic, antioxidant properties, and can repair damage produced by alloxan toxicity.