Studies on Decolourization of Textile Effluent Using Bacillus subtilis and Pseudomonas aeruginosa Isolated from Textile Effluent

Abstract

Studies on Decolourization of Textile Effluent Using Bacillus subtilis and Pseudomonas aeruginosa Isolated from Textile Effluent Aim: This research work aimed to Study the decolourization of Textile Effluent Using Bacillus subtilis and Pseudomonas aeruginosa isolated From Textile Effluent. Study Design: This study was designed to isolate and identify Bacillus Subtilis and Pseudomonas aeruginosa from textile effluent. To use bacterial isolates in decolourization of the textile effluent individually and in the consortium (both isolates together) at three different concentrations. Place and Duration of Study: Department of Microbiology, Nasarawa State University Keffi, Nasarawa State, Nigeria, between August 2016 and November 2017. Methodology: Textile effluent was collected in a screw-capped sterilized bottle from the textile mill discharge point. The bacterial species of choice were isolated from the textile effluent and were subjected to bacterial decolorization individually and then in consortium using a decolorization medium, composed of minimal salt medium and textile effluent for a period of 12 days, the %Decolorization was measured by checking absorbance of the sample at 72hours intervals using a spectrophotometer (user 720 nm wavelength). This was repeated at different concentrations of 20 ml/250 ml, 20 ml/500 ml, and 20 ml/750 ml (v/v of textile effluent and minimal salt medium). Results: Bacillus subtilis and Pseudomonas aeruginosa were found to have significant potential to decolourize the dye effluent. Bacillus subtilis produced high decolourization activity after 12 days with 58.78% at 20 ml/750 ml concentration. Whereas, Pseudomonas aeruginosa produced 53.46% at 20 ml/750 ml concentration after 12 days period of incubation. Pseudomonas aeruginosa and Bacillus subtitles in consortium produced the highest decolourization potential of 75.56% at concentration 20 ml/750 ml after 12 days period of incubation. Conclusion: This study shows that Bacillus subtilis have comparatively higher decolourization potential than Pseudomonas aeruginosa. The bacteria in consortium exhibited the highest ability in the decolorizing process than the individual isolates. However, there is a need for further work to be done to validate and improve these findings.