Optimization of Extraction Procedure for Isolation, Identification & Quantification of Piperine from Roots of Piper longum Linn.

Abstract

Optimization of Extraction Procedure for Isolation, Identification & Quantification of Piperine from Roots of Piper longum Linn. Aim: Piperine which is mainly found in the fruits and roots of the Piper longum plant and is a medicinally active bio-molecular compound, used as potential ingredient in preparation of several medicines. This present study was carried out to optimize suitable extraction process  for evaluation of piperine from the roots of Piper longum. Study design:  Root samples were collected from three different areas of Odisha during the month of August-October 2013. Harvested roots were shade dried and pulverized into fine particles for extraction procedure. Place and Duration of Study: The total experimentation was carried out at Seed Bank and Seed Biology Division, Regional Plant Resource Centre during the month of October 2013 to          February 2014. Methodology: Piperine had been extracted through various solvent systems and different extraction procedures viz. water bath and cold stirring method from the roots of P. longum. Isolated extracts were analyzed through spectrophotometric and high performance liquid chromatography (HPLC) method. Results: In case of spectrophotometric analysis, piperine content was found within a range of 0.243-0.525% dry weight when extracted through water bath using methanol and ethanol. In case of cold stirrer method, using methanol: water & ethyl acetate: water, piperine content was ranged from 0.208-0.443% dry weight. Through HPLC method, piperine content was found within 0.21-0.49% dry weight when extracted through water bath and 0.156-0.4% dry weight when extracted through cold stirring method. Conclusion: According to these findings it could be opined that the roots possessed a remarkable amount of piperine. Samples collected from the Keonjhar showed higher piperine as compared to G Udayagiri and Khurda regions. However optimization of extraction methods along with utilization of solvent system prevailed Water bath method to be superior to cold stirring process and methanol was found to be efficient solvent system for extracting higher amount of piperine.