In vitro Anticoagulant Effect of Grape Seed Extract (Vitis vinifera) on Human Normal Blood Sample

Abstract

Background: Scientists have used herbs nowadays for curing many diseases because they are safer and to overcome the side effect of the chemical drugs. Grape is one of the ancient herbs that used for diseases of the heart and blood vessels, high blood pressure, high cholesterol, skin care and many other conditions. Grape seeds have significantly higher concentrations of polyphenols which has anticoagulant and antithrombotic effect. Materials and Methods: In this study 20 normal blood samples from healthy individuals with age range (19-38) years were enrolled in this study]. Prothrombin time (PT) and activated partial thromboplastin time (APTT) tests were performed before adding grape seed extraction (GSE) (as control) and after adding GSE with different concentrations (25%, 50% and 75%). Results: The results revealed that grape seed extract has an anticoagulant effect as proven by the increase of Prothrombin time and Activated partial thromboplastin time results of the blood samples in different concentrations of the extract. The GSE showed a high statistical significant (P= 0.000) in all concentrations of both PT and APTT tests. Conclusion: This study suggests that GSE has a strong anticoagulant effect; so it can potentially be used as a supplementary anticoagulant agent to prevent thrombosis and cardiovascular diseases.