Next-Generation Sequence: A Review on Metagenomic Approach to Discovery of Novel Enzymes from the Soil Environment
Asian Journal of Biotechnology and Bioresource Technology · pp. 10–19 · Published 30 Mar 2021
10.9734/ajb2t/2021/v7i130091Abstract
Next-generation sequencing (NGS) makes a large mass of sequences. As a technology that allows the sequence of deoxyribonucleic acid (DNA) molecules larger than one million base pairs, it has been applied in the food research and medical fields. In the food sector, NGS has been used in food safety for the detection of species authenticity of food products and for mostly discovering novel industrial enzymes. The soil ecosystem houses a great number of non-culturable microbes thus novels enzymes can still be discovered to date. The conventional methods used in enzyme discovery have less chances to identify novel gene clusters and bioactivities. Therefore, there is a dire need for high-throughput technology, together with advanced bioinformatics for the search of novel enzymes or biocatalysts from soil metagenomes. This review article thus gives a summary of the progress in the application of next-generation sequencing in the identification and characterization of novel enzymes with a special focus on enzymes from the soil environment.
References (25)
- 1 Genome sequencing in microfabricated high-density picolitre reactors [DOI]
- 2 Accurate whole human genome sequencing using reversible terminator chemistry [DOI]
- 3 An integrated semiconductor device enabling non-optical genome sequencing [DOI]
- 4 Molecular biological access to the chemistry of unknown soil microbes: a new frontier for natural products [DOI]
- 5 Cloning the Soil Metagenome: a Strategy for Accessing the Genetic and Functional Diversity of Uncultured Microorganisms [DOI]
- 6 Real-Time DNA Sequencing Using Detection of Pyrophosphate Release [DOI]
- 7 Enzyme immobilization: an update [DOI]
- 8 Metagenomic Analyses: Past and Future Trends [DOI]
- 9 Library construction for next-generation sequencing: Overviews and challenges [DOI]
- 10 Total bacterial diversity in soil and sediment communities—A review [DOI]
- 11 Massively Parallel Sequencing: The Next Big Thing in Genetic Medicine [DOI]
- 12 Construction and analysis of bacterial artificial chromosome libraries from a marine microbial assemblage [DOI]
- 13 Accessing the Soil Metagenome for Studies of Microbial Diversity [DOI]
- 14 A Review on the Applications of Next Generation Sequencing Technologies as Applied to Food-Related Microbiome Studies [DOI]
- 15 Screening for novel lipolytic enzymes from uncultured soil microorganisms [DOI]
- 16 Are Uncultivated Bacteria Really Uncultivable? [DOI]
- 17 Characterization of a Forest Soil Metagenome Clone That Confers Indirubin and Indigo Production on Escherichia coli [DOI]
- 18 Evaluation of a Transposase Protocol for Rapid Generation of Shotgun High-Throughput Sequencing Libraries from Nanogram Quantities of DNA [DOI]
- 19 Metagenomic applications in exploration and development of novel enzymes from nature: a review [DOI]
- 20 Isolation of high molecular weight DNA from soil for cloning into BAC vectors [DOI]
- 21 Activity screening of environmental metagenomic libraries reveals novel carboxylesterase families [DOI]
- 22 A new esterase EstD2 isolated from plant rhizosphere soil metagenome [DOI]
- 23 Characterization of Novel Polycyclic Aromatic Hydrocarbon Dioxygenases from the Bacterial Metagenomic DNA of a Contaminated Soil [DOI]
- 24 Identification and Characterization of Lipolytic Enzymes from a Peat-Swamp Forest Soil Metagenome [DOI]
- 25 Discovery of two novel β-glucosidases from an Amazon soil metagenomic library [DOI]
Cited by 0
No indexed citations yet.
Related research
- A Brief Review of Oyster-associated Microbiota — shares topic coverage
- Pivotal Role of High Sensitivity Variant Calls and Confirmation Methods for Next-Generation Sequencing Findings: A Case Report — shares topic coverage
- From Antigens to Alleles: Evolving Strategies in Blood Group Typing — shares topic coverage
- Spider Species Identification: Bridging Traditions with Molecular and Deep Learning Approaches — shares topic coverage
- Coffin-Siris Syndrome Confirmed by an ARID1B Mutation: A Case Report — shares topic coverage
Article metrics
Real usage data collected on this platform.
38
Page views
0
PDF downloads
5
Outbound clicks
0
Citations
Views over time
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
Traffic sources
Referring site, by host.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.