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Neem Leaf Supplement Ameliorates Depressive Like Behaviour in Alzheimer’s Disease Model in Adult Male Wistar Rats

Adejoke Elizabeth Memudu, Fatima Ayinda Anzaku, Grace Mchibuma Jibaniya, Rukky Precious Adanike

Asian Journal of Research in Medical and Pharmaceutical Sciences · pp. 31–46 · Published 24 Jan 2024

10.9734/ajrimps/2024/v13i1245

Abstract

Aim of the Study: Currently, reports linked neuropathological changes in Alzheimer’s Disease (AD) to be a risk factor in depression. There is a need to develop natural therapeutics, with strong antioxidant property like Neem leaf, to avert AD’s neuropathological and behavioural changes using animal model induced by neurotoxin aluminium chloride. This study is aimed at ascertaining depressive like disorders in AD model while evaluating mechanism through which Neem averts AD neurodegeneration in the fronto-cerebellar cortex and possible depressive like behaviour. Methodology: Twenty (20) adult male Wistar rats used were grouped (n=5) viz: control group (A), neurodegenerative model given aluminium (B), 200mg/kg Neem leaf supplement (C) and 200mg/kg Neem leaf treated AD model (D). Neurobehavioural changes for reward memory and depressive like behaviour were evaluated using Y-maze (open arm reward test) and the tail suspension test (TST).  The frontal and cerebellar cortices were excised, fixed and processed for  Haematoxylin and Eosin stain (H and E), Cresyl fast violet (CFV) stain for Nissl bodies and astrocytes immunohistochemistry using glial fibrillary acidic protein (GFAP). Behavioural test data were analyzed using ANOVA and test for significance done @ p<0.05. Results: Aluminum induced neurodegeneration similar to AD pathology characterized by loss of neurons, chromatolysis, proliferation of astrocytes and decline in cognitive function in addition to exhibiting depressive like behaviour seen in a decrease number of reward arm entries, increase in time spent to reach reward arm and immobility time in the TST attributed to loss of cognitive function relative to loss of neurons integrity in the fronto-cerebellar cortex. However, neem leaf supplementation mitigates against AD model neuropathological and neurobehavioural  presentations resulting in an improved neurocognition, neuron survival or repair, decline in astrocytes proliferation and decline in depressive like behavior as compared to Aluminum induced AD model. Conclusion: Neem leaf alleviates depressive like behaviour associated with neurocognitive impairment through the interaction between neuron-astrocytes to protect neurons against aluminum induced neuroinflamamation and strengthens neural circuit for cognitive function.

Depression astrocytes chromatolysis neem leaf supplement neuroinflammation Alzheimer's disease memory impairment fronto-cerebellar cortex

References (32)

  1. 1 Theory and Practice of Histological Techniques [DOI]
  2. 2 The tail suspension test as a model for assessing antidepressant activity: Review of pharmacological and genetic studies in mice [DOI]
  3. 3 Guide for the Care and Use of Laboratory Animals [DOI]
  4. 4 N-Acetylcysteine as an antioxidant and disulphide breaking agent: the reasons why [DOI]
  5. 5 Therapeutics Role of Azadirachta indica (Neem) and Their Active Constituents in Diseases Prevention and Treatment [DOI]
  6. 6 The Tail Suspension Test [DOI]
  7. 7 Guidelines for the Care and Use of Mammals in Neuroscience and Behavioral Research [DOI]
  8. 8 Reactive Astrocytes in Neurodegenerative Diseases [DOI]
  9. 9 Depression as a Risk Factor for Alzheimer’s Disease: A Systematic Review of Longitudinal Meta-Analyses [DOI]
  10. 10 Mediterranean diet adherence, gut microbiota, and Alzheimer's or Parkinson's disease risk: A systematic review [DOI]
  11. 11 Astrocyte, a Promising Target for Mood Disorder Interventions [DOI]
  12. 12 Dietary Patterns and Alzheimer’s Disease: An Updated Review Linking Nutrition to Neuroscience [DOI]
  13. 13 Stress-Induced Depression and Alzheimer’s Disease: Focus on Astrocytes [DOI]
  14. 14 Dietary carotenoids related to risk of incident Alzheimer dementia (AD) and brain AD neuropathology: a community-based cohort of older adults [DOI]
  15. 15 Neurological disorders in autism: A systematic review and meta-analysis [DOI]
  16. 16 Ethnobotanical uses of neem (Azadirachta indica A.Juss.; Meliaceae) leaves in Bali (Indonesia) and the Indian subcontinent in relation with historical background and phytochemical properties [DOI]
  17. 17 Elevated brain aluminium and early onset Alzheimer’s disease in an individual occupationally exposed to aluminium: a case report [DOI]
  18. 18 Elevated Plus Maze and Y-Maze Behavioral Effects of Subchronic, Oral Low Dose Monosodium Glutamate in Swiss Albino Mice [DOI]
  19. 19 Role of aqueous extract of Azadirachta indica leaves in an experimental model of Alzheimer′s disease in rats [DOI]
  20. 20 Aluminum Induced Immunoexcitotoxicity in Neurodevelopmental and Neurodegenerative Disorders [DOI]
  21. 21 Astrocytes in depression and Alzheimer’s disease [DOI]
  22. 22 Impaired Cognitive Flexibility and Working Memory Precedes Depression: A Rat Model to Study Depression [DOI]
  23. 23 Memory complaints and depressive symptoms over time: a construct-level replication analysis [DOI]
  24. 24 Association between dementia and depression: a retrospective study using the Korean National Health Insurance Service-National Sample Cohort database [DOI]
  25. 25 Antibacterial Effect of Azadirachta indica (Neem) or Curcuma longa (Turmeric) against Enterococcus faecalis Compared with That of 5% Sodium Hypochlorite or 2% Chlorhexidine in vitro [DOI]
  26. 26 Agmatine as a novel candidate for rapid-onset antidepressant response [DOI]
  27. 27 Alpha lipoic acid ameliorates scopolamine induced memory deficit and neurodegeneration in the cerebello-hippocampal cortex [DOI]
  28. 28 Cognitive profiles in persons with depressive disorder and Alzheimer’s disease [DOI]
  29. 29 Aluminum may mediate Alzheimer’s disease through liver toxicity, with aberrant hepatic synthesis of ceruloplasmin and ATPase7B, the resultant excess free copper causing brain oxidation, beta-amyloid aggregation and Alzheimer disease [DOI]
  30. 30 A Study of Some Effects of Aqueous Extract of Neem (Azadirachta Indica) Leaves on the Lead Acetate Induced Neurotoxicity in the Superficial Layers of Superior Colliculus of Adult Wistar Rats (Rattus norvegicus) [DOI]
  31. 31 Correlation between Dementia and Depressive Disorder [DOI]
  32. 32 Astrocyte-neuron interplay is critical for Alzheimer’s disease pathogenesis and is rescued by TRPA1 channel blockade [DOI]

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