Hepatoprotective Effect of Origanum elongatum against Carbon Tetrachloride (CCl4) Induced Toxicity in Rats

Abstract

Aims: This study was undertaken to investigate the potential effect of methanol extract of Origanum elongatum (OEME) given orally by gavage against intraperitoneal injection a single dose of Carbon tetrachloride (CCl4)(0.6 ml/kg) induced hepatotoxicity in rats. Study Design: Biochemical analysis, histological examination and in vivo study. Place and Duration of Study: Laboratory of Biology and health (Faculty of Science), between June 2012 and August 2012. Methodology: Hepatoprotective activity of OEME at four doses of 250 mg/kg, 500 mg/kg, 1000 mg/kg and 2000 mg/kg body weight. The degree of protection was estimated by biochemical analysis of serum liver biomarkers: AST, ALT, ALP and by liver histopathological examination. Results: The total phenolic content of OEME (83.61 ± 0.19 mg AGE / g extract) and total flavonoid content (10.85 ± 0.05 mg QE / g extract) were found significantly high. The present study showed that OEME was dose dependently decreased hepatic histopathological changes and serum liver biomarkers levels in CCl4 intoxicated rats. Conclusion: Data from present results revealed the hepatoprotection of OEME against hepatotoxic products or drugs.