Development of Yellow Mosaic Virus Resistance in Mung Bean through EMS Mutagenesis

Abstract

Green gram is a variety of mungbean (Vigna radiata) and one of the most crucial food legumes consumed as a protein source. One of the most destructive diseases of the legume is Yellow mosaic virus. It is caused by bipartite begomoviruses namely mungbean yellow mosaic virus (MYMV). One of the ways to strengthen resistance against the virus is mutation breeding. Several programs of induced mutations have been conducted in the subcontinent successfully. Ethyl methanesulfonate (EMS) been used as a chemical mutagen in the TILLING technique for mutation breeding programs for a while now since it induces point mutation within the genome. In this experiment the seeds of BARI Mung-6 were treated with six different concentrations (0.05%, 0.1%, 0.15%, 0.20%, 0.3% and 0.4%) EMS. Seeds were grown and advanced in the greenhouse till M3 generation. Individual lines were chosen as per standard disease grade 0-3. The M4 generation was grown in the research field and 13 lines were chosen as per level of disease resistance and 100 seed weight. Three lines were identified as high yielding resistant material from those thirteen tested lines.