Morphogenotyping of Cyclosa bifida (Arachnida: Araneidae) from Odisha, India

Abstract

Morphogenotyping involves analyzing an individual's morphology and DNA sequences to identify structural and genetic differences. Among the arthropod group, the spider is the most diversified, primarily female, and solely predatory. The surveys were carried out in the morning, from 5:00 AM to 8:00 AM, and in evening from 5:00 PM to 6:00 PM, well before darkness. With the help of identification keys and based on morphological features, all collected spiders were identified and made taxonomical positions. Then DNA was isolated from the single female captured C. bifida species. Quality was evaluated on 0.8 % Agarose Gel; a single band of high-molecular weight DNA has been observed. Isolated DNA was amplified with cytochrome oxidase subunit I (COI) gene Specific Primer (LCO 1490 and Chelicerate Reverse 2). The consensus sequence was generated   for each sample using BioEdit version 6.0.7 and were searched over the GenBank database using Basic Local Alignment Search Tool (BLAST) against the spider genomes previously presentin GenBank. The complete sequences were deposited in GenBank with accession no. PQ478048.1. This morphogenotyping investigation will demonstrate the need for additional research on several unidentified or perplexed spiders. Thus, research suggests that the bifida species found in West Bengal and Odisha are more genetically similar and closer to one another than those found in Kerala. These discrepancies would result from two distinct evolutionary pathways.