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Research Article Open access CC BY 4.0

Molecular Epidemiology of Shiga Toxin-Producing Escherichia coli Isolated from School Children in Ondo State, Nigeria

A. K. Onifade, M. A. Oladoja

Microbiology Research Journal International · pp. 340–347 · Published 5 Feb 2015

10.9734/BMRJ/2015/14535

Abstract

Molecular techniques were used for studying the epidemiology of Escherichia coli from five major towns in Ondo state, namely; Akure and Ondo, Owo, Okiutipupa and Ikare-Akoko. In total, 807 urine and faecal samples of apparently healthy primary school pupils were gathered between December 2012 and March 2013. Withal, 206 E. coli strains were isolated and characterized by biochemical tests. Polymerase chain reaction (PCR) assay was performed to determine the presence of the genes-encoding virulence factors. A total of 22 (12 male, 10 female) isolates out of 28 subjected to PCR amplification were found to possess only stx1 virulence gene associated with Enterohaemorrhagic E. coli strains. PCR assay was demonstrated to be a useful technique for the epidemiological work of E. coli where this organism is a major cause of infection.

Epidemiology Escherichia coli shiga toxin urine faecal PCR

References (20)

  1. 1 Diarrheagenic Escherichia coli [DOI]
  2. 2 District Laboratory Practice in Tropical Countries [DOI]
  3. 3 Pathogenesis and Diagnosis of Shiga Toxin-Producing Escherichia coli Infections [DOI]
  4. 4 Enterohaemorrhagic Escherichia coli : emerging issues on virulence and modes of transmission [DOI]
  5. 5 Escherichia colifrom animal reservoirs as a potential source of human extraintestinal pathogenicE. coli [DOI]
  6. 6 Molecular Epidemiology: Focus on Infection [DOI]
  7. 7 Incidence of Enterohemorrhagic Escherichia coli, Escherichia coli O157, Salmonella, and Listeria monocytogenes in Retail Fresh Ground Beef, Sprouts, and Mushrooms [DOI]
  8. 8 Evidence of septic system failure determined by a bacterial biochemical fingerprinting method [DOI]
  9. 9 Increased human pathogenic potential of Escherichia coli from polymicrobial urinary tract infections in comparison to isolates from monomicrobial culture samples [DOI]
  10. 10 Serogroups, virulence genes and antibiotic resistance in Shiga toxin-producing Escherichia coli isolated from diarrheic and non-diarrheic pediatric patients in Iran [DOI]
  11. 11 EnteropathogenicEscherichia coliAssociated with Diarrhea in Children in Cairo, Egypt [DOI]
  12. 12 Characterization of Urinary Tract Infection-Associated Shiga Toxin-Producing Escherichia coli [DOI]
  13. 13 Identification & characterization of Shiga toxin-producing Escherichia coli isolates from patients with diarrhoea in Iran.
  14. 14 Molecular Epidemiological Analysis of Escherichia coli Isolates Producing Extended-Spectrum β-Lactamases for Identification of Nosocomial Outbreaks in Stockholm, Sweden [DOI]
  15. 15 Study Prevalence of Verotoxigenic E.coli Isolated from Urinary Tract Infections (UTIs) in an Iranian Children Hospital [DOI]
  16. 16 Multiple drug resistance of enteropathogenic Escherichia coli isolated from children with diarrhea in Kashan, Iran [DOI]
  17. 17 Prevalence and characterization of Shiga toxin O157 and non-O157 enterohemorrhagic Escherichia coli isolated from different sources in Ismailia, Egypt [DOI]
  18. 18 Two cases of urinary tract infection caused by Shiga toxin-producing Escherichia coli O157:H7 strains.
  19. 19 Genotypes and phenotypes of Shiga toxin-producing Escherichia coli (STEC) in Abeokuta, Southwestern Nigeria [DOI]
  20. 20 Prevalence Of Enterohaemorrhagic Eschericia Coli 0157:H7 Causing Severe Urinary Tract Infection In Abeokuta, Nigeria

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