Molecular Characterization and Antimicrobial Susceptibility Pattern of Microorganisms from Port Harcourt Correctional Center, Rivers State Nigeria
T. Sampson, I.C. Ohanekwu, L.P. Peekate
Asian Journal of Research in Infectious Diseases · pp. 29–41 · Published 10 May 2024
10.9734/ajrid/2024/v15i5348Abstract
Correctional centers have been reported to be associated with several conditions predisposing inmates to health challenges as a result of exposure to disease causing microbial population. This study therefore targeted the Molecular Characterization and Antimicrobial susceptibility pattern of Microorganisms from Port Harcourt Correctional Center, Rivers State Nigeria. A total of 150 samples (100 from skin (upper arm) and 50 from wall surfaces) were collected using sterile swab sticks and immediately transported to Department of Microbiology Laboratory, Rivers State University for analyses, using standard microbiological techniques. Molecular characterization was done using Polymerase Chain reaction (PCR) and subjected to gene sequencing. The Kirby-Bauer Disc Diffusion method was used for the antimicrobial susceptibility test. Well in agar method was used to assess the susceptibility of the isolates to honey and Aloe Vera extract. The study revealed that 100% of all the bacterial isolates were resistant to Meropenem, Vancomycin, cefuroxime, and Ceftazidine, while 90% of the bacterial isolates were susceptible to Gentamicin, Cepftriaxone, Erythromycin, and Ciproflozaxine. Data showed the MAR index ranged from 0.3 to 0.8. Statistical analysis revealed there were no significant differences in the mean values of data obtained from the animate and inanimate surfaces (p > 0.05). Molecular identification confirmed the isolates to be Kocuria palustris, Serretia liquefaciens, Klebsiella pneumonia, Enterococcus feacalis, Bacillus flexus, and Aspergillus niger. The results revealed that honey was more effective (100%) than Aloe Vera extract which inhibited only one of the Escherichia coli isolates. The study displayed the antimicrobial effect of honey against both Gram positive and Gram negative bacterial isolates. While adequate personal hygiene, proper sanitation, and spaced room should be encouraged, honey can be used in correctional centers to control bacterial skin infections.
References (10)
- 1 The multifaceted roles of antibiotics and antibiotic resistance in nature [DOI]
- 2 The antibacterial activities of honey [DOI]
- 3 The perfect storm: incarceration and the high-risk environment perpetuating transmission of HIV, hepatitis C virus, and tuberculosis in Eastern Europe and Central Asia [DOI]
- 4 Preparation of lactophenol cotton blue slide mounts.
- 5 An Assessment of Prison Overcrowding in Nigeria: Implications for Rehabilitation, Reformation and Reintegration of Inmates. [DOI]
- 6 Amplification of Bacterial 16S Ribosomal DNA with Polymerase Chain Reaction [DOI]
- 7 Molecular Characterization and Detection of Antibiotic Resistance Genes in Pseudomonas Species Isolated from Tympanotonus fuscatus [DOI]
- 8 Molecular Characterisation and Antibiogram of Vaginal Flora from Students Attending a Tertiary Institution in Port Harcourt, Rivers State, Nigeria [DOI]
- 9 Antibiogram and Multiple Antibiotics Resistance Indices of Bacterial Isolates from Urine of Diabetics and Nondiabetics Attending Some Hospitals in Rivers State, Nigeria [DOI]
- 10 Microbiological Survey of Animate and Inanimate Surfaces in a Correctional Center, South-South, Nigeria [DOI]
Cited by 0
No indexed citations yet.
Related research
- Bacteriological and Fungal Evaluation of Three Brands of Disinfectants Sold in Calabar Municipality, Cross River State — shares topic coverage
- Seroprevalence of Hepatitis B and Listeriosis Co-Infection among Patients Attending Sickbay Hospital, Ahmadu Bello University, Zaria, Kaduna, Nigeria — shares topic coverage
- Prevalence and Susceptibility Analysis of Carbapenem Resistant Gram-negative Pathogens in Super Specialty Tertiary Care Center, Mumbai, India — shares topic coverage
- Serological Characterization and Antimicrobial Sensitivity Profile of Haemophilus influenzae Serotypes Isolated from Aminu Kano Teaching Hospital, Kano, Nigeria — shares topic coverage
- Microbiology of Chronic Otitis Media — shares topic coverage
Article metrics
Real usage data collected on this platform.
52
Page views
0
PDF downloads
26
Outbound clicks
0
Citations
Views over time
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
Traffic sources
Referring site, by host.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.