Protective Properties of Citrullus lanatus on Carbon Tetrachloride Induced Liver Damage in Rats

Abstract

Aim: This study was conducted to investigate the effects of the leaf extract of Citrullus lanatus on carbon tetrachloride (CCl4) induced liver damage in rats. Methodology: Hepatoprotective study was conducted using five groups (A-E) of six male rats per group. Groups A and B served as the control groups and were respectively administered with distilled water and CCl4 in olive oil while groups C, D and E were administered respectively with 500, 1,000 and 1,500mg/kg body weight of the ethanolic leaf extract of C. lanatus for 7 days. Animals were subsequently anaesthetized, blood samples were collected for alanine amino transferase (ALT), aspartate amino transferase (AST), alkaline phosphatase (ALP), total bilirubin, total protein and albumin assays; liver organ was isolated and processed for biochemical and histopathological studies. Results: There were significant increases (P=0.05) in the serum levels of ALT and AST both in the plasma and liver homogenate in the group treated with CCl4 as compared to negative control group; while in the extract treated group, there was a decrease in the levels of the aminotransferases. Histopathological studies showed severe portal congestion and necrosis of hepatocytes in the group treated with CCl4 while the groups administered with the extract treated group showed a moderate venous congestion with absence of necrosis. Conclusion: These results highlight the ability of the leaf extract of C. lanatus to ameliorate damage in the liver of albino rats.