Microbial Contamination and Antibiotic Resistance in Enteric Pathogens Isolated from Cooked Foods Sold in Eateries in Ado-Ekiti, Nigeria
Microbiology Research Journal International · pp. 236–246 · Published 26 Jan 2015
10.9734/BMRJ/2015/7351Abstract
Background: There are indications that some ready-to-eat or street vended foods are of low microbial quality with varying degree of contaminations. The contaminants may include antibiotic-resistant bacteria that may further be spread through consumption of such foods which has epidemiological and public health implications. This study therefore, was aimed at investigating the microbial quality of ready-to-eat cooked foods sold in eateries in Ado-Ekiti, Nigeria and to determine the susceptibility of the isolates to antibiotics. Methods: We evaluated bacterial contamination of ready-to-eat cooked foods using total bacteria plate counts (TPC) and total coliform counts (TCC). Susceptibility of isolates to antibiotics was tested using the disc diffusion method. Results: The overall mean TPC and TCC of cooked food samples ranged from 4.96±1.01 log10 cfu/g to 5.34±0.06 log10 cfu/g for both. There was no significant difference (≤ 0.05) in the overall mean TPC and TCC of the various cooked food samples from the three categories of sampling sites. The cooked foods examined were contaminated with a total of 129 enteric bacterial species belonging to 7 genera with E. coli (31.8%) being the most prevalent, followed by Klebsiella sp. (19.4%), Proteus sp. (17.1%), Salmonella (14.0%), Pseudomonas sp. (12.4%), Shigella sp. (3.8%) and Enterobacter sp. (1.6%) in that order. Resistance to antibiotics was very high (94.0%) and resistance to amoxicillin (89.1%) was the highest, and least to nalidixic acid (23.1%) while none of the isolates was resistant to ofloxacin. More than 50% of these isolates were resistant to six of the eight antibiotics tested and 26 different antibiotic resistance phenotypes were obtained. The most common phenotype observed was AMX/GEN/AUG. The results suggest that contaminated ready-to-eat (RTE) food sold in eateries can be a major source of antibiotic-resistant organisms in the study area. Conclusion: Most of the food samples investigated was contaminated in varying degrees with pathogenic, opportunistic pathogenic bacteria and other bacterial indicators of contamination. There was an overall high rate of resistance to antibiotics with equally high rate of multiple antibiotic resistance (MAR) among the bacterial contaminants. This portent high risks of further spread of antibiotic resistance in the community and environment. Further study will hopefully help to determine the presence of resistance genes and or mode of resistance among the MAR organisms.
References (33)
- 1 Antimicrobial Resistance in the Food Chain: A Review [DOI]
- 2 The survival and transfer of microbial contamination via cloths, hands and utensils [DOI]
- 3 Acquired antibiotic resistance in lactic acid bacteria from food [DOI]
- 4 Antimicrobial Resistance of Escherichia coli O157 Isolated from Humans, Cattle, Swine, and Food [DOI]
- 5 The emergence and spread of antibiotic resistance in food-borne bacteria [DOI]
- 6 Colonization factors of Campylobacter jejuni in the chicken gut [DOI]
- 7 Antimicrobial resistance of Escherichia coliisolates from broiler chickens and humans [DOI]
- 8 Antibiotic Resistance in Food-Borne Bacterial Contaminants in Vietnam [DOI]
- 9 Hygienic status assessment of dish washing waters, utensils, hands and pieces of money from street food processing sites in Ouagadougou (Burkina Faso) [DOI]
- 10 Evaluation of Standard- and High-Dose Daptomycin versus Linezolid against Vancomycin-Resistant Enterococcus Isolates in an In Vitro Pharmacokinetic/Pharmacodynamic Model with Simulated Endocardial Vegetations [DOI]
- 11 Detection of Shiga Toxin-Producing Escherichia coli Serotypes O26:H11, O103:H2, O111:H8, O145:H28, and O157:H7 in Raw-Milk Cheeses by Using Multiplex Real-Time PCR [DOI]
- 12 Spread of antibiotic resistance with food-borne pathogens [DOI]
- 13 Antimicrobial susceptibility of Enterobacteriaceae isolated from vegetables [DOI]
- 14 Food Safety and Hyienic Practices of Street Food Vendors in Owerri, Nigeria [DOI]
- 15 Variation in Acid Resistance among Shiga Toxin-Producing Clones of Pathogenic Escherichia coli [DOI]
- 16 A study of microbial safety of ready-to-eat foods vended on highways: Onitsha-Owerri, south east Nigeria
- 17 THE OCCURRENCE, GROWTH AND CONTROL OF PATHOGENS IN AFRICAN FERMENTED FOODS [DOI]
- 18 Frequency of indicator bacteria, Salmonella and diarrhoeagenic Escherichia coli pathotypes on ready-to-eat cooked vegetable salads from Mexican restaurants [DOI]
- 19 Microbiological quality assessment of foods sold in student's cafeterias
- 20 Food consumption patterns in the Waterloo Region, Ontario, Canada: a cross-sectional telephone survey [DOI]
- 21 Incidence of Antibiotic Resistance in Some Bacterial Pathogens from Street Vended Food in Ogbomoso, Nigeria [DOI]
- 22 Trends in Susceptibility of Vancomycin-Resistant Enterococcus faecium to Tigecycline, Daptomycin, and Linezolid and Molecular Epidemiology of the Isolates: Results from the Tigecycline In Vitro Surveillance in Taiwan (TIST) Study, 2006 to 2010 [DOI]
- 23 Occurrence And Antimicrobial Drug Resistance Of Potential Bacterial Pathogens From Shellfish, Including Queen Conchs (Strombus Gigas) And Whelks (Cittarium Pica) In Grenada
- 24 Survival of bacterial enteric pathogens in traditional fermented foods [DOI]
- 25 EXAMINATION OF THE MICROBIAL STATUS OF SELECTED INDIGENOUS FERMENTED FOODS IN NIGERIA [DOI]
- 26 Antibiotic resistance in Escherichia coli isolated from healthy cattle at a major cattle market in Ibadan, Oyo State, South Western, Nigeria [DOI]
- 27 Source(s) of contamination of raw and ready-to-eat foods and their public health risks in Mekelle City, Ethiopia [DOI]
- 28 RESIDUES OF STREPTOMYCIN ANTIBIOTIC IN MEAT SOLD FOR HUMAN CONSUMPTION IN SOME STATES OF SW NIGERIA RESIDUOS DE ESTREPTOMICINA EN CARNE EN SW NIGERIA
- 29 Bacteriological quality of weaning food and drinking water given to children of market women in Nigeria: implications for control of diarrhoea.
- 30 Smarter inspection will improve food safety in Canada [DOI]
- 31 Antimicrobial Use and Resistance in Africa [DOI]
- 32 Studies on foodborne bacteria in commercially hawked ready-to-eat fish in Jos and its environments [DOI]
- 33 Fishes and smoked meat delicacies as sources of multidrug resistant bacteria and parasitic worms [DOI]
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