Metallo-Beta-Lactamase Producing Isolates of Escherichia coli and Klebsiella pneumoniae and their Resistance Profiles in Enugu,  Nigeria: A Threat to Public Health

Uzoamaka Charity Maduakor; Clara Idara Eleazar; Chidi George Mba; Chiamaka Cynthia Obodochukwu; Chioma Lily Eberechukwu; Cornelius Osinachi Ogu

doi:10.9734/jamb/2024/v24i2791

Research Article Open access CC BY 4.0

Metallo-Beta-Lactamase Producing Isolates of Escherichia coli and Klebsiella pneumoniae and their Resistance Profiles in Enugu, Nigeria: A Threat to Public Health

Uzoamaka Charity Maduakor, Clara Idara Eleazar, Chidi George Mba, Chiamaka Cynthia Obodochukwu, Chioma Lily Eberechukwu, Cornelius Osinachi Ogu

Journal of Advances in Microbiology · pp. 11–19 · Published 9 Feb 2024

10.9734/jamb/2024/v24i2791

Abstract

Background: A potential threat to public health is the rapidly spreading enterobacteriaceae, especially Escherichia coli and Klebsiella pneumoniae which produce metallo-beta-lactamases (MBL). This study evaluated the prevalence of metallo-beta-lactamase (MBL) from clinical and non-clinical sources in Enugu Metropolis. Methodology: The study was conducted in the Microbiology Laboratory of the University of Nigeria Teaching Hospital, Ituku-Ozalla between October 2020 and July 2021. A total of 150 isolates including 85 and 65 isolates of Escherichia coli and Klebsiella pneumoniae respectively was recovered. Standard microbiology procedures were used to identify and characterize the isolates. Antimicrobial susceptibility was done using the Kirby-Bauer disc diffusion technique. Phenotypic detection of Metallo-beta-lactamase production was determined using Combined Disk Tests. Results: Imipenem resistance was detected in 22 (25.9%) isolates of E. coli and 18 (27.7%) isolates of K. pneumoniae. Of the 22 strains of E. coli that were imipenem resistant, 8 (9.4%) and 14 (16.5%) were found to be MBL producers and non-MBL respectively. Of the 18 strains of Klebsiella pneumoniae that were imipenem resistant, 10 (15.4%) were MBL producers and 8 (12.3%) were non-MBL producers. The highest prevalence of MBL was recovered from urine sources in both E. coli and K. pneumonieae. All MBL-producing isolates were multidrug resistant. Conclusion: The overall prevalence of MBL in this study was 12.0%. Public health is at risk due to the occurrence of metallo-beta-lactamase. Antimicrobial stewardship and the implementation of infection control strategies are required to halt the spread of these resistant bacteria in the environment. The use of antibiotics should be with utmost prudence.

Antibiotic resistance carbapenemase metallo-beta-lactamase phenotypic detection Escherichia coli Klebsiella pneumoniae

References (20)

1 Emergence of a new antibiotic resistance mechanism in India, Pakistan, and the UK: a molecular, biological, and epidemiological study [DOI]
2 Carbapenem resistance in Enterobacteriaceae: here is the storm! [DOI]
3 Past and Present Perspectives on β-Lactamases [DOI]
4 Phenotypic Detection of Carbapenemase-Producing Organisms from Clinical Isolates [DOI]
5 The Current Burden of Carbapenemases: Review of Significant Properties and Dissemination among Gram-Negative Bacteria [DOI]
6 Extended spectrum beta-lactamase and metallo beta-lactamase production among Escherichia coli and Klebsiella pneumoniae isolated from different clinical samples in a tertiary care hospital in Kathmandu, Nepal [DOI]
7 The Association Between Colonization With Carbapenemase-Producing Enterobacteriaceae and Overall ICU Mortality [DOI]
8 β-lactam resistance associated with β-lactamase production and porin alteration in clinical isolates of E. coli and K. pneumoniae [DOI]
9 Metallo-beta-lactamase producing Escherichia coli and Klebsiella pneumoniae: A rising threat for hospitalized children.
10 Detection of Beta-Lactamases (ESBL and MBL) Producing Gram-Negative Pathogens in National Public Health Laboratory of Nepal [DOI]
11 Utilisation des carbapénèmes en pédiatrie [DOI]
12 Detection of Various Beta-Lactamases in Escherichia coli and Klebsiella sp.: A Study from Tertiary Care Centre of North India [DOI]
13 DETECTION OF METALLO-?-LACTAMASESENCODING GENES AMONG CLINICAL ISOLATES OF ESCHERICHIA COLI IN A TERTIARY CARE HOSPITAL, MALAYSIA [DOI]
14 Phenotypic study of Extended-spectrum beta-lactamase, AmpC and Carbapenemase among E.coli clinical isolates in affiliated hospitals of Babol University of Medical Sciences [DOI]
15 Phenotypic detection of metallo-beta-lactamase production in clinical isolates of Escherichia coli and Klebsiella pneumoniae in a tertiary care hospital [DOI]
16 Phenotypic detection of ESBL and MBL in Gram Negative bacilli isolated from clinical specimens [DOI]
17 International Journal of Current Microbiology and Applied Sciences [DOI]
18 JOURNAL OF CLINICAL AND DIAGNOSTIC RESEARCH [DOI]
19 Journal of the Pakistan Medical Association
20 Journal of Medical Science and Clinical Research [DOI]

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Uzoamaka Charity Maduakor, Clara Idara Eleazar, Chidi George Mba, Chiamaka Cynthia Obodochukwu, Chioma Lily Eberechukwu, Cornelius Osinachi Ogu (2024). Metallo-Beta-Lactamase Producing Isolates of Escherichia coli and Klebsiella pneumoniae and their Resistance Profiles in Enugu, Nigeria: A Threat to Public Health. Journal of Advances in Microbiology , 11–19. https://doi.org/10.9734/jamb/2024/v24i2791

Affiliations

Uzoamaka Charity Maduakor — Department of Medical Laboratory Sciences, Faculty of Health Sciences and Technology, College of Medicine, University of Nigeria, Nigeria.
Clara Idara Eleazar — Department of Microbiology, Faculty of Biological Sciences, University of Nigeria, Nsukka, Nigeria.
Chidi George Mba — College of Medicine, University of Nigeria, Enugu Campus, Nigeria.
Chiamaka Cynthia Obodochukwu — Department of Medical Laboratory Sciences, Faculty of Health Sciences and Technology, College of Medicine, University of Nigeria, Nigeria.
Chioma Lily Eberechukwu — Department of Medical Laboratory Sciences, Faculty of Health Sciences and Technology, College of Medicine, University of Nigeria, Nigeria.
Cornelius Osinachi Ogu — Department of Medical Laboratory Sciences, Faculty of Health Sciences and Technology, College of Medicine, University of Nigeria, Nigeria.

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