Male Pheromone Isolation, Chemical Analysis and the Perfumes Development from Goat Hair Waste from Some Local Farms in Thailand

Abstract

  Goat male pheromones are secreted through their hair and play a prominent role in “male effect”. In this study, we aimed to isolate some male pheromone from the goat hair, waste from local goat farm from the Southern Thailand. Goat hairs were collected from three farms in three breeds and different parts of goat bodies compared in both males and females. The goat hairs extraction was carried out by maceration with hexane. The isolation of the extract was performed by column chromatography using sephadex LH-20 to give 100 fractions. The further separations yielded the 4-ethyloctanoic acid reported as goat scent and the exert releaser pheromone activity to attract females. Quantitative analyses using GC-MS demonstrated 12.73±0.78% w/w in the extract. The results showed 17 fatty acid compositions, such as palmitic acid, stearic acid, cis-9-octadecenomic acid, lauric acid, cis-vaccenic acid as prominent compounds. The qualitative of the extract by GC-MS illustrated 13 compounds in which the chemical fingerprint was confirmed by using 1H NMR technique. The microbial contamination of goat hair extract was further evaluated by spread-plate technique, showing no microbial contamination with Staphylococcus aureus, Clotridium spp. and Salmonella spp. The extract was used as a raw material for perfumes blended development and no irritation on human skin was observed.