Skip to content
Research Article Open access CC BY 3.0

Listeria spp. and Enterobacteriaceae Group in Sandwiches of Meat and Meat Products

Rashed A. Zaghloul, Moustafa A. El-Shenawy, Naseem A. Neweigy, Hamed E. Abou-Aly, Raouf K. El-dairouty, Wagih I. El-Kholy, Mohamed T. Fouad, J. M. Soriano, J. Mañes, Lydia Micó

Microbiology Research Journal International · pp. 360–368 · Published 3 Jan 2014

10.9734/BMRJ/2014/7417

Abstract

Aims: To evaluate the Egyptian street-vended sandwiches of meat and meat products for the presence of Listeria monocytogenes and Enterobacteriaceae group. To evaluate the microbiological quality of street-vended sandwiches of meat and meat products sold in Cairo-Egypt. Place and Duration of Study: Department of Food Microbiology, National Research Center, Cairo, at during the period of January 2011 to September 2012. Methology: Seventy sandwiches of meat and meat products including ten samples each of burger, hawawshi, kofta, liver, luncheon, sausages and shawerma sandwiches were randomly collected from the street-vendors and food shops in Great-Cairo governorate. Samples were investigated for their loads of Enterobacteriaceae counts as well as the presence of L. monocytogenes. Enterobacteriaceae counts was done using the conventional International method (FDA), however isolation and identification of L. monocytogenes was carried out using three different microbiological examination methods including classic selective conventional media, chromogenic media, as well as rapid identification method “food-system kits”. 168 isolates of Listeria spp were identified following the biochemical identification tests (Bergey’s Manual) and confirmed using Hi Listeria identification kits and latex test kits. Results: Enterobacteriaceae group were detected in 51% of the examined samples with an average counts of 37x102 cfu/g. Listeria spp. were detected in range between 24% and 36%, depended on the method used, with numbers ranged from 16x102 to 23x102 cfu/g. All samples that were contaminated with Listeria spp. harboured L. monocytogenes. Listeria spp. was not detected in all the examined Hawawshi sandwiches with an exception of one positive sample detected using the chromogenic media. The obtained results indicated that 37 out 68 (54%) isolates, picked up from classic selective conventional media, and 62 out of 100 (62%) isolates from chromogenic medium were confirmed as L. monocytogenes indicating that chromogenic medium may be the superior for isolation of the pathogen from ready-to-eat sandwiches of meat and products. Conclusion: The obtained results indicated that these foods presented a source of infection to the consumer. Measures to control the quality of the raw material, environmental and hygienic conditions during preparation and serving should be taken. The chromogenic media was the most efficient for the isolation of the pathogen during this course of study.

Meat meat products sandwiches Enterobacteriaceae Listeria monocytogenes.

References (24)

  1. 1 Compendium of Methods for The Microbiological Examination of Foods [DOI]
  2. 2 Antimicrobial-Resistant Pathogens Associated With Healthcare-Associated Infections: Annual Summary of Data Reported to the National Healthcare Safety Network at the Centers for Disease Control and Prevention, 2006–2007 [DOI]
  3. 3 Compendium of Methods for the Microbiological Examination of Foods [DOI]
  4. 4 Antimicrobial-Resistant Pathogens Associated with Healthcare-Associated Infections Summary of Data Reported to the National Healthcare Safety Network at the Centers for Disease Control and Prevention, 2009–2010 [DOI]
  5. 5 Mandell, Douglas, and Bennett’s Principles and Practice of Infectious Diseases [DOI]
  6. 6 Microbiological Quality and Safety of Ready-to-Eat Street-Vended Foods in Johannesburg, South Africa [DOI]
  7. 7 The incidence of significant foodborne pathogens in domestic refrigerators [DOI]
  8. 8 The street food trade in Africa: safety and socio-environmental issues [DOI]
  9. 9 Listeria monocytogenes in Raw Milk: Detection, Incidence, and Pathogenicity [DOI]
  10. 10 A selective differential medium for the isolation of Listeria monocytogenes [DOI]
  11. 11 Inactivation of Escherichia coli O157:H7, Salmonella enterica Serotype Enteritidis, and Listeria monocytogenes on Lettuce by Hydrogen Peroxide and Lactic Acid and by Hydrogen Peroxide with Mild Heat [DOI]
  12. 12 Effect of mild heat pre-treatment with alkaline electrolyzed water on the efficacy of acidic electrolyzed water against Escherichia coli O157:H7 and Salmonella on Lettuce [DOI]
  13. 13 New chromogenic plating media for detection and enumeration of pathogenic Listeria spp.—an overview [DOI]
  14. 14 Critical Control Points of Street-Vended Foods in the Dominican Republic [DOI]
  15. 15 Evaluation of chromogenic media for the detection of Listeria species in food [DOI]
  16. 16 Culture media and methods for the isolation of Listeria monocytogenes [DOI]
  17. 17 Toxinfecçöes alimentares: uma revisäo
  18. 18 Multicenter Study of Street Foods in 13 Towns on Four Continents by the Food and Environmental Hygiene Study Group of the International Network of Pasteur and Associated Institutes [DOI]
  19. 19 Microbiological quality of hamburgers sold in the streets of Cuiabá - MT, Brazil and vendor hygiene-awareness [DOI]
  20. 20 Culture Media and Methods for the Isolation of Listeria monocytogenes [DOI]
  21. 21 Principles and Practice of Infectious Diseases. [DOI]
  22. 22 Food and Drug Administration [DOI]
  23. 23 Bergey's Manual [DOI]
  24. 24 Food Service at Temporary Events and Casual Public Gatherings [DOI]

Cited by 23

Environmental and seasonal drivers of microbiological contamination in street-vended foods

Christelle F. Iskandar, Reine Abi Khalil, Nour Gereige · Scientific Reports · 2025

Microbiological safety of ready-to-eat foods in low- and middle-income countries: A comprehensive 10-year (2009 to 2018) review.

Oluwadamilola M. Makinde, K. Ayeni, M. Sulyok · Comprehensive Reviews in Food Science and Food Safety · 2020

Assessment of microbiological quality of ready to eat meat sandwiches in new valley governorate

S. Sotohy, Esraa Mohamed, Ashraf Abd El-malek · 2019

Current Trend on the Economic and Public Health Significance of Salmonellosis in Iraq

N. Abdulhaleem, B. Garba, H. Younis · Advances in Animal and Veterinary Sciences · 2019

Caracterización del consumo de comida callejera según edad, estudio multicéntrico

S. Durán-Agüero, L. Arboleda, John Edinson Velásquez Vargas · Revista Española de Nutrición Humana y Dietética · 2018

Street food research worldwide: a scoping review

K. Abrahale, S. Sousa, G. Albuquerque · Journal of human nutrition and dietetics (Print) · 2018

Street foods in Beirut city: An assessment of the food safety practices and of the microbiological quality

Myriam Loukieh, E. Mouannes, C. A. Jaoudeh · 2018

Rutin inhibits mono and multi-species biofilm formation by foodborne drug resistant Escherichia coli and Staphylococcus aureus

N. A. Al-Shabib, F. Husain, I. Ahmad · 2017

Effect of thyme extract on some Enterobacteriaceae isolated from some meat products in Assuit city

S. F. Elhawary, R. Hassanein, M. Agban · 2016

Risk Factors and Outcomes of Food Poisoning in Africa

N. Malangu · 2016

Showing 16 of 23 known citations — external sources report more than can currently be individually listed.

Related research

Article metrics

Real usage data collected on this platform.

117

Page views

0

PDF downloads

58

Outbound clicks

23

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.