LC-ESI/MS and GC-MS Methanol Extract Analysis, Phytochemical and Antimicrobial Activity Studies of Centella asiatica
Daniel A. Ondeko, Bernard F. Juma, Lilechi D. Baraza, Peter K. Nyongesa
Asian Journal of Chemical Sciences · pp. 32–51 · Published 7 Oct 2020
10.9734/ajocs/2020/v8i319046Abstract
Aims: To determine chemical constituents of the Leaf extracts of Centella asiatica using the LC-MS and GC-MS and their antimicrobial activities. Study Design: Structural determination of compounds from the leaf extracts was done using GC-MS and LC-MS analysis. The antimicrobial properties of the extracts were done using disc diffusion method. Place and Duration of Study: Pure and Applied Chemistry Department, Masinde Muliro University of Science and Technology, Kenya: Between 2016-2019. Methodology: Plant materials of C. asiatica were sequentially extracted separately based on the polarity viz., hexane, ethyl acetate and methanol. Determination of chemical constituents was done using LC-MS and GC-MS analysis and phytochemical screening. The extracts were assayed against Staphylococcus aureus, Klebsiella pneumonia, Escherichia coli and Candida albicans. Standard antimicrobials viz. ampicillin and Nystatin were used as the control. Disc diffusion method was used and zones of inhibition, after respective incubation periods, were used to quantify antibacterial and antifungal activity. Results: Phytochemical screening of the hexane, ethyl acetate and methanolic extracts showed that terpenoids, flavonoids, saponins, alkaloids, steroids, amino acids and proteins, reducing sugars and carbohydrates were present. LC-MS and GC-MS analyses of the methanolic extracts identified 22 and 33 compounds, respectively, by use of the national institute of standards and technology (NIST) library. The extracts showed appreciable activity against common microbes tested. Conclusion: This study forms the basis for the biological characterization and significance of the compounds identified in the leaf and stem extracts of C. asiatica. These compounds are known to possess antibacterial and antifungal activities that could be established as potential candidates for future drug development. However, these extracts, need to be subjected to further chromatographic procedures to isolate the identified compounds and their bioactivities determined.
References (32)
- 1 Phytochemical analysis of some medicinal plants
- 2 Pharmacological review on Centella asiatica: A potential herbal cure-all [DOI]
- 3 Principles of assessing bacterial susceptibility to antibiotics using the agar diffusion method [DOI]
- 4 Chemical, pharmacological and clinical profile of the East Asian medical plant Centella aslatica [DOI]
- 5 In vitro and in vivo wound healing activity of asiaticoside isolated from Centella asiatica [DOI]
- 6 Pentacyclic Triterpenoids from the Medicinal Herb, Centella asiatica (L.) Urban [DOI]
- 7 Centella asiatica(L.) Urban: From Traditional Medicine to Modern Medicine with Neuroprotective Potential [DOI]
- 8 Determination of biologically active constituents in Centella asiatica [DOI]
- 9 GC-MS analysis of phytocomponents in the ethanol extract of Polygonum chinense L. [DOI]
- 10 Evaluation of anxiolytic properties of Gotukola – (Centella asiatica) extracts and asiaticoside in rat behavioral models [DOI]
- 11 A REVIEW ON MEDICINAL PROPERTIES OF CENTELLA ASIATICA [DOI]
- 12 The healing effects of Centella extract and asiaticoside on acetic acid induced gastric ulcers in rats [DOI]
- 13 The Anti-thrombotic Active Constituents from Centella asiatica [DOI]
- 14 Chemical Composition and Antibacterial Activity of the Essential Oil ofCentella asiatica. Growing in South Africa [DOI]
- 15 An improved HPLC method for quantitative determination of six triterpenes in Centella asiatica extracts and commercial products.
- 16 Identification of triterpenoid compounds of Centella asiatica by thin‐layer chromatography and mass spectrometry [DOI]
- 17 Identification and quantification of triterpenoid centelloids inCentella asiatica(L.) Urban by densitometric TLC [DOI]
- 18 Chemical constituents ofCentella asiatica [DOI]
- 19 Comparison of antibiotic discs from different sources. [DOI]
- 20 Notes on the occurrence and significance of triterpenoids (asiaticoside and related compounds) and caffeoylquinic acids in Centella species [DOI]
- 21 Integration of mass spectral fingerprinting analysis with precursor ion (MS1) quantification for the characterisation of botanical extracts: application to extracts of Centella asiatica (L.) Urban [DOI]
- 22 Analysis of Biologically Active Constituents in Centella asiatica by Microwave-Assisted Extraction Combined with LC–MS [DOI]
- 23 EVALUATION OF EFFECT OF CENTELLA ASIATICA ON CCL4 INDUCED RAT LIVER DAMAGE
- 24 Two new flavonoids from Centella asiatica (Linn.) [DOI]
- 25 Serotypes and anti-microbial susceptibility of Plesiomonas shigelloides isolates from humans, animals and aquatic environments in different countries [DOI]
- 26 Irbic acid, a dicaffeoylquinic acid derivative from Centella asiatica cell cultures [DOI]
- 27 Coumestrol inhibits autoantibody production through modulating Th1 response in experimental autoimmune thyroiditis [DOI]
- 28 The effect of the phytoestrogen coumestrol on the NZB/W F1 murine model of systemic lupus [DOI]
- 29 Essential Oil Profiling of Centella asiatica (L.) Urb.-A Medicinally Important Herb [DOI]
- 30 LCMS/MS metabolite profiling and analysis of acute toxicity effect of the ethanolic extract of Centella asiatica on zebrafish model
- 31 Lupus Erythematosus and Herbal Medicine [DOI]
- 32 Traditional Asian medicine and leprosy in Bangladesh [DOI]
Cited by 25
Waqas Razzaq, Jean Baptiste Mazzitelli, Anne Sylvie Fabiano Tixier · Molecules · 2026
Ethel Jeyaseela Jeyaraj, Sheila Nathan, Yau Yan Lim · Access Microbiology · 2022
Suproteem Mukherjee, Avijit Chakraborty, Indranil Santra · 3 Biotech · 2025
T. Premkumar, S. Sajitha Lulu · Journal of Biomolecular Structure and Dynamics · 2024
Anbazhakan Kandasamy, Kanakaraj Aruchamy, Praveena Rangasamy · Plants · 2023
Lúcio Ricardo Leite Diniz, Leonardo Luiz Calado, Allana Brunna Sucupira Duarte · Metabolites · 2023
Zaw Myo Hein, Prarthana Kalerammana Gopalakrishna, Anil Kumar Kanuri · Life · 2025
Claude Y. Hamany Djande, Paul A. Steenkamp, Ian A. Dubery · Phyton · 2025
Siti-Zaharah Rosli, Noranizan Mohd Adzahan, Roselina Karim · Molecules · 2022
Kamana Sharma, Manisha Bhusal, Akash Budha Magar · PLOS One · 2025
Showing 11 of 25 known citations — external sources report more than can currently be individually listed.
Related research
- Characterization and Evaluation of the Antibacterial Activity of Silver Nanoparticles Synthesized by Green Method Using Phyllanthus emblica Leaf Extract — shares topic coverage
- Antibacterial and Phytochemical Activity of Gliricidia sepium against Poultry Pathogens — shares topic coverage
- Evaluation of Antibacterial Activity of Piper betel Leaves and Nigella sativa Seeds against Multidrug Resistant Food and Water Borne Pathogenic Bacteria: An in vitro Study Model — shares topic coverage
- Traditional and Molecular Identification of Bacterial Contamination from Honey (Processed and Unprocessed) and Their Control by Phytotherapy — shares topic coverage
- Antibacterial, Antioxidant Activity and Phytochemical Analysis of Euphorbia hirta Linn. — shares topic coverage
Article metrics
Real usage data collected on this platform.
51
Page views
0
PDF downloads
15
Outbound clicks
25
Citations
Views over time
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
Traffic sources
Referring site, by host.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.