Phytochemical Evaluation and Effect of Saponins’ Mixture Isolated from Astragalus monspessulanus on HepG2 Cell Line
Viktor Bratkov, Magdalena Kondeva-Burdina, Rumyana Simeonova, Virginia Tzankova, Ilina Krasteva
European Journal of Medicinal Plants · pp. 522–527 · Published 20 Jan 2014
10.9734/EJMP/2014/7860Abstract
Aims: Purified saponin fraction was obtained from butanol extract of the aerial parts of Astragalus monspessulanus var. monspessulanus. The fraction was analyzed by HPLC and six saponins were determined. The saponin mixture was investigated for possible cytotoxicity on HepG2 cell line. Study Design: HPLC, in vitro study, biochemical analysis - lactate dehydrogenase leakage in medium Place and Duration of Study: Laboratory of Drug metabolism and drug toxicity, between September and November 2013. Methodology: The saponin mixture was studied on HepG2 cell line in 3 different concentrations –1, 2, 4 mg/ml for 24, 48 and 72 hours. The release of lactate dehydrogenase in the medium was measured spectrophotometrically. Results: It was found that the saponins have statistically significant cytotoxicity only in the highest concentration 4 mg/ml on 48 and 72 hours. At this concentration the sample increased the level of LDH (lactate dehydrogenase) with 37 % on 48 h and with 52 % on 72 h. Conclusion: Purified saponin fraction, isolated from Astragalus monspessulanus was found to be cytotoxic at the highest concentration 4 mg/ml on 48 and 72 hours in HepG2 cell line.
Cited by 5
5 citations reported by external sources — individual citing-article records aren't available to list yet.
Related research
- Molecular Identification, Bioactivity Screening and Metabolic Fingerprinting of the Actinomycetes of Chenab River Sediments — shares topic coverage
- Antimalarial Efficacy of Bergenia ciliata (Saxifragaceae) Leaf Extract In vitro against Plasmodium falciparum and In vivo against Plasmodium berghei — shares topic coverage
- Anti-viral Activity Evaluation of Selected Medicinal Plants of Nigeria against Measles Virus — shares topic coverage
- In Vitro Antioxidant and In Vivo Antidiabetic Properties of Citrus Maxima Leaf Extracts in Alloxan-Induced Swiss Albino Diabetic Mice — shares topic coverage
- Cytotoxic Effects of Cadmium and Paraquat on Avian Skin Fibroblasts — shares topic coverage
Article metrics
Real usage data collected on this platform.
0
Page views
0
PDF downloads
0
Outbound clicks
5
Citations
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
No views recorded yet.
Traffic sources
Referring site, by host.
No traffic recorded yet.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.