Effect of 1,5-Bis (3,5-dimethylpyrazol-1-yl)-3-oxapentane Diacetatocopper on Kidney and Spleen of Rats: Histological and Immunohistochemical Evaluation

Abstract

The present work was carried out to evaluate the histological and immunohistochemical changes of the kidney and spleen of rats after repeated exposure to 1,5-Bis (3,5-dimethylpyrazol-1-yl)-3-oxapentane diacetatocopper (BDO). Two groups of male albino rats were used. The first group is the control and the second group was i.p. given 1,5-Bis(3,5-dimethylpyrazol-1-yl)-3-oxapentane diacetatocopper (12 mg / kg body weight) daily for 6 weeks. The results showed that the administration of BDO to rats exhibits various histological effects on tissues of kidney and spleen. Kidney from treated animals showed congestion of blood vessels, degeneration of renal tubules and glomeruli. The spleen tissue lost its characteristic structure and showing interference \ overlap between red and white pulp, enlarged trabecula and degenerated lymphocytes. Histochemical results revealed depletion of total proteins contents in renal tubules and glomeruli and spleen cells of treated rats. Expression of Bcl-2 protein was increased in the kidney and spleen of treated rats. It is concluded from the present work that BDO induced histopathological alterations and apoptosis in kidney and spleen of rats.