CRISPR-Cas Mediated Genome Editing: A Paradigm Shift towards Sustainable Agriculture and Biotechnology
Rao Saad Rehman, Syed Ali Zafar, Mujahid Ali, Asad Nadeem Pasha, Muhammad Saqib Naveed, Muhammad Waseem, Muhammad Ahmad, Asad Raza
Asian Plant Research Journal · pp. 27–49 · Published 28 Jan 2022
10.9734/aprj/2022/v9i130197Abstract
CRISPR–Cas genome editing technology developed from prokaryotes has transformed the molecular biology of plants past all assumptions. CRISPR–Cas, which is distinguished by its resilience, relatively high specificity, and easy implementation, enables specific genetic modification of crops, allowing for the creation of germplasms with favorable characters and the development of innovative, highly efficient agricultural systems. Moreover, many new biotechnologies in the framework of CRISPR–Cas platforms have bolstered basic research as well as synthetic biology toolkit of plants. In this article, initially, we provide a brief overview of CRISPR–Cas gene editing, emphasis on the modern, most specific gene-editing techniques, such as prime and base editing. Following that, the major role of CRISPR–Cas in plants in enhancing pesticide and disease resistance, quality, yield, breeding, and faster domestication are next discussed. In this review, we discuss the current advancements in plant biotechnology linked to CRISPR–Cas, such as CRISPR–Cas gene control, reagent conveyance, multiplexed gene editing, directed evolution, and mutagenesis. In the end, we talk about how this innovative technology may be used in the future.
Cited by 12
Hidam Bishworjit Singh, Niraj Kumar, Riwandahun Marwein · Genome and Epigenome Editing for Stress‐Tolerant Crops · 2025
Fasiha Qurashi, Syed Riaz Ahmed, Muhammad Yousaf Shani · CRISPRized Horticulture Crops · 2024
Ilya Zlobin, Marina Efimova, Natalia Permykova · Physiology · 2023
Natalya V. Permyakova, Elena V. Deineko · Horticulturae · 2024
Kamati N. Teresia, Samuel C. Chukwu, Pamwenafye I. Nanhapo · Advances in Agriculture · 2025
Kamel A. Abd-Elsalam, Aftab Ahmed, Ahmad Munir · CRISPRized Horticulture Crops · 2024
Zunnorain Ali, Zakir Ullah, Javed Iqbal · CABI Biotechnology Series · 2025
Anuj Kumar Poonia, Bhavika Batra, Milan Kumar Lal · Global Regulatory Outlook for CRISPRized Plants · 2024
Showing 8 of 12 known citations — external sources report more than can currently be individually listed.
Related research
- Non-invasive Ultra-early in Utero Detection and Precision CRISPR-mediated Correction of Monogenic Embryonic Mutations: A Critical Appraisal of a Hypothetical Therapeutic Framework — shares topic coverage
- Precision Genetic Engineering for Crop Improvement in Modern Agriculture: A Critical Review — shares topic coverage
- Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)–CRISPR–Cas Systems: Mechanisms, Genome Editing Applications, Therapeutic Potential, and Ethical Considerations — shares topic coverage
- Genomic-Assisted Breeding Tools for Grain and Nutritional Quality Improvement in Rice — shares topic coverage
- Contemporary Trends and Novelties in Biotechnology: From Programmable Biology to Scalable Translation — shares topic coverage
Article metrics
Real usage data collected on this platform.
0
Page views
0
PDF downloads
0
Outbound clicks
12
Citations
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
No views recorded yet.
Traffic sources
Referring site, by host.
No traffic recorded yet.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.