Isolation and Screening of Amylase from Moulds Associated with the Spoilage of Some Fermented Cereal Foods
A. M. Omemu, G. Bamigbade, A. O. Obadina, T. M. Obuotor
Microbiology Research Journal International · pp. 359–367 · Published 6 Nov 2014
10.9734/BMRJ/2015/11190Abstract
Aims: The aim of the study is to isolate moulds from spoilt samples of three fermented cereal foods (ogi, eko, pap) and screen the mould for amylase production. Study Design: Primary screening for amylase production by the mould isolates was determined on starch agar. Isolates having the widest zones of hydrolysis were further screened by Solid state fermentation [SSF] Methodology: Molds were isolated on Sabouraud Dextrose Agar [SDA] incubated at 28°C±2°C for 72h and identified by standard microbiological methods. Screening of moulds for amylase activity was determined on starch agar medium and flooed with gram’s iodine. The isolates were screened quantitatively for the production of α-amylase and glucoamylase using Solid state fermentation [SSF] while the enzyme activities were determined spectrophotometrically. Results: Mean mould population observed on the spoilt food samples ranged from 5.06 log cfu/g for ogi to 5.80 log cfu/g for eko wrapped in leaves. Twenty seven moulds isolated from the samples were identified as Aspergillus flavus, Aspergillus niger, Aspergillus fumigatus, Penicillium citrinum and Rhizopus stolonifer. Majority (70.4%) of the isolated mould produced amylase on starch agar indicated by clear zones around the colonies. Diameter of the zone of hydrolysis ranged from 3.0 mm for Aspergillus niger EL4 to 22.0 mm for Aspergillus flavus OG1. The highest amylase activity was 30.8% from A. flavus OGI while the least was 21.2% from A. flavus EN4. Glucoamylase activities observed were between 100 U/ml and 240 U/ml. respectively. Conclusion: This study contributes to catalogued local fungal isolates that can be used for amylase production and provides additional information to support future research about the industrial enzymes potential of these microorganisms. More work is however needed to determine the optimal production conditions of the enzymes.
References (25)
- 1 Fungi and Food Spoilage [DOI]
- 2 The Paleozoic Origin of Enzymatic Lignin Decomposition Reconstructed from 31 Fungal Genomes [DOI]
- 3 New developments in solid state fermentation: I-bioprocesses and products [DOI]
- 4 Solid state fermentation for the production of industrial enzymes
- 5 Food-processing enzymes from recombinant microorganisms—a review [DOI]
- 6 The influence of nitrogen sources on the α-amylase productivity of Aspergillus oryzae in continuous cultures [DOI]
- 7 Bacteriocinogenic activity of lactobacilli from fermented sausages [DOI]
- 8 Raw starch degrading amylase production by mixed culture of Aspergillus niger and Saccharomyces cerevisae grown on sorghum pomace [DOI]
- 9 Physical and nutritive properties of fermented cereal foods [DOI]
- 10 PRODUCTION OF AMYLASE BY A. NIGER IN A CHEAP SOLID MEDIUM USING RICE BRAN AND AGRICULTURAL MATERIALS
- 11 Structure of the complex of a yeast glucoamylase with acarbose reveals the presence of a raw starch binding site on the catalytic domain [DOI]
- 12 Industrialization of Ogi Fermentation [DOI]
- 13 Isolation and Screening of Amylolytic Filamentous Fungi [DOI]
- 14 The fate of aflatoxin B1 in the production of ogi, a Nigerian fermented sorghum porridge [DOI]
- 15 Novel uses for lactic acid fermentation in food preservation [DOI]
- 16 Isolation and Toxicity of Molds from Foods Stored in Homes [DOI]
- 17 Amylolytic potentiality of fungi isolated from some Nigerian agricultural wastes [DOI]
- 18 Yeasts and Moulds Associated with ogi-A Cereal Based Weaning Food During Storage [DOI]
- 19 Spoilage of Some Stored Fermented Foods in South West Nigeria [DOI]
- 20 History of the Study of Thrombolytic and Fibrinolytic Enzymes of Higher Basidiomycetes Mushrooms at the V.L. Komarov Botanical Institute in Saint Petersburg, Russia [DOI]
- 21 Production of thermostable amylolytic enzymes by Aspregillus niger F-909 under solid state fermentation
- 22 BIOUTILIZATION OF ADANSONIA DIGITATA FRUIT PULP BY BACILLUS SPECIESFOR AMYLASE PRODUCTION
- 23 Amylase Production by Rhizopus nigricans Using Mashed Maize [DOI]
- 24 Amylase Recovery From Mouldy Bran Of Aspergillus niger AM07 [DOI]
- 25 Statistical Analysis of Growth and Amylase Production by Aspergillus flavus grown on Different Carbon Sources [DOI]
Cited by 5
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