Isolation and Identification of Staphylococci and Pseudomonas from Diabetic Foot Ulcer in Perambalur District Hospitals in Tamil Nadu

Abstract

Diabetic foot ulcers are still a problem for the health care system and are majorly influencing quality of life. Infected foot ulcer is one of the most feared complications of diabetes mellitus, leading to gangrene and it needs to be amputed. Diabetic foot lesions are major medical, social and economic problem and leading cause of hospitalization for patients with diabetes worldwide. The risk of a diabetic patient for developing a foot ulcer was estimated to be ~25%. Samples were collected from 65 infected patients of diabetic foot ulcer male and female. The mean age group was found to be 40-80 years with the clinical history such as age, sex, types of diabetes duration of diabetes, size of ulcer and duration of ulcer were observed and recorded. The Specific mediums were used for the isolation and identification using classical methods based on their morphology, Grams stain reaction, oxidase and catalase tests were performed to confirm the isolates were both gram-negative and gram-positive organisms Bergey’s manual of Determinative Bacteriology. Further, MALDI- TOF (Matrix-Assisted Laser Desorption/Ionization) was used to confirm the isolates identified in classical methods were Staphylococci (79%) and Pseudomonas (21%) In this study Among 68 isolates, identified in out of 41 isolates Pseudomonasaeruginosa (21%) Staphylococcus aureus (54%) Staphylococcus hominis (20%)and Staphylococcous hemolytic(5%). Although in gram positive organism Staphylococcus aureus (54%) was the most predominant isolate found in diabetic foot ulcer.