Influence of Urine on Antimicrobial Gradient and Disk Diffusion Susceptibility Testing of  Co-trimoxazole, Fosfomycin,  Norfloxacin and Nitrofurantoin  against Urinary Escherichia coli

Anju Kuriakose; Arathy Krishna; Gopika Raj R G; Sruthi P; Divya Das; Anila Gopal; Innu S; Roopa Mathews; Harish Kumar K S

doi:10.9734/jamb/2024/v24i2797

Research Article Open access CC BY 4.0

Influence of Urine on Antimicrobial Gradient and Disk Diffusion Susceptibility Testing of Co-trimoxazole, Fosfomycin, Norfloxacin and Nitrofurantoin against Urinary Escherichia coli

Anju Kuriakose, Arathy Krishna, Gopika Raj R G, Sruthi P, Divya Das, Anila Gopal, Innu S, Roopa Mathews, Harish Kumar K S

Journal of Advances in Microbiology · pp. 74–85 · Published 27 Feb 2024

10.9734/jamb/2024/v24i2797

Abstract

Aim: The aim of present study is to examine the influence of urine on zone of inhibition and Minimum inhibitory concentration by antimicrobial gradient strips of Co-trimoxazole, Fosfomycin, Norfloxacin and Nitrofurantoin against urinary E. coli. Study Design: Cross- sectional study. Place and Duration of Study: Department of Medical Microbiology, School of Medical Education, Kottayam, Kerala, India. Between January 2023 and November 2023. Methodology: A total of 75 E. coli isolates collected from various diagnostic microbiology laboratories were included in the study. Identification of isolates and antimicrobial susceptibility testing was done. Direct v/s standard zone of inhibition and MIC was determined. The data was statistically analysed using Cohen’s kappa for interrater reliability and Intraclass correlation coefficient for consistency level. Results: Current study evaluated the effect of urine on zone of inhibition and MIC by disc diffusion and antibiotic gradient testing respectively. The kappa value, a measure of agreement between direct v/s standard zone of inhibition and MIC for Co-trimoxazole, Fosfomycin, Norfloxacin was a perfect 1 (P=.000), indicating complete agreement. While, The kappa value, a measure of agreement between direct and standard MIC determination for Nitrofurantoin, was .67 (P=.000), indicating substantial agreement. Conclusion: The present study suggest that direct antimicrobial susceptibility testing can be employed for Co-trimoxazole, Fosfomycin, Norfloxacin and Nitrofurantoin which are important drugs in the management of UTI, after further standardization.

Escherichia coli co-trimoxazole fosfomycin norfloxacin nitrofurantoin direct sensitivity testing antimicrobial susceptibility testing urinary tract infection

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Anju Kuriakose, Arathy Krishna, Gopika Raj R G, Sruthi P, Divya Das, Anila Gopal, Innu S, Roopa Mathews, Harish Kumar K S (2024). Influence of Urine on Antimicrobial Gradient and Disk Diffusion Susceptibility Testing of Co-trimoxazole, Fosfomycin, Norfloxacin and Nitrofurantoin against Urinary Escherichia coli. Journal of Advances in Microbiology , 74–85. https://doi.org/10.9734/jamb/2024/v24i2797

Affiliations

Anju Kuriakose — Department of Medical Microbiology, School of Medical Education, Center for Professional and Advanced studies, Kottayam, Kerala, India.
Arathy Krishna — Department of Medical Microbiology, School of Medical Education, Center for Professional and Advanced studies, Kottayam, Kerala, India.
Gopika Raj R G — Department of Medical Microbiology, School of Medical Education, Center for Professional and Advanced studies, Kottayam, Kerala, India.
Sruthi P — Department of Medical Microbiology, School of Medical Education, Center for Professional and Advanced studies, Kottayam, Kerala, India.
Divya Das — Department of Medical Microbiology, School of Medical Education, Center for Professional and Advanced studies, Kottayam, Kerala, India.
Anila Gopal — Department of Medical Microbiology, School of Medical Education, Center for Professional and Advanced studies, Kottayam, Kerala, India.
Innu S — Department of Medical Microbiology, School of Medical Education, Center for Professional and Advanced studies, Kottayam, Kerala, India.
Roopa Mathews — Department of Medical Microbiology, School of Medical Education, Center for Professional and Advanced studies, Kottayam, Kerala, India.
Harish Kumar K S — Department of Medical Microbiology, School of Medical Education, Center for Professional and Advanced studies, Kottayam, Kerala, India.

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