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Research Article Open access CC BY 4.0

Influence of Cultural Media, Temperature, and pH on the Growth Characteristics of Alternaria brassicicola Isolated from Cauliflower

Kalpna, Vijay Kumar, Gurusimran Kaur, Anuj Prasad

Journal of Scientific Research and Reports · pp. 826–832 · Published 14 Nov 2025

10.9734/jsrr/2025/v31i113713

Abstract

The genus Alternaria is among the most predominant plant pathogens, recognised as a major constraint to crop productivity extending from 10–70% in India and a catastrophic cause of yield losses among brassicaceous crops. The experiment was conducted to assess the in-vitro effects of cultural and physiological variations on mycelial growth of Alternaria brassicicola (Schwein.) Wiltshire during 2023-2024. Cultural studies indicated that among the six different tested media, Potato dextrose agar supported the maximum mycelial growth (86.48 mm), whereas Czapek’s dextrose dox agar media supported the minimum growth (36.91 mm). For the maximum growth of the fungus, the best temperature observed was 25 °C (82.24 mm), while minimum growth was recorded at 35 °C (11.14 mm). Among the five different pH levels tested, the pathogen showed optimum mycelial growth at pH 5 (81.57 mm), while the least growth was recorded at pH 8 (41.45 mm).

Alternaria brassicicola black leaf spot culture media isolation pH temperature

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