Skip to content
Research Article Open access CC BY 4.0

Induction and Expression of Chitinases from Four Sub Species of Bacillus thuringiensis

P. W. H. K. P. Daulagala

Journal of Advances in Microbiology · pp. 1–8 · Published 29 May 2017

10.9734/JAMB/2017/34084

Abstract

Chitin is a naturally occurring linear polymer of N-acetylglucosamine and the major structural component of fungal cell walls and exoskeletons of insects and arthropods. Chitinases are the enzymes that breakdown chitin to economically important derivatives and found in a range of organisms including bacteria, insects, crustaceans, invertebrates, some vertebrates and higher plants. In the present study, four Bacillus thuringiensis (Bt) isolates were grown in media supplemented with 0.1% (w/v) regenerated chitin and chitinase inducing medium and  screened for chitinolytic activity. All isolates showed notable extracellular chitinase activity with very low levels in cell bound fractions. When the isolates were screened with fluorogenic substrates, Bt HD133, Bt T7002 and Bt IPS78 showed a similar pattern of distribution with the highest production from Bt IPS 78. The lowest activity was detected from the isolate Bt 36-3. Similar to the results obtained with fluorogenic substrates, Bt 36-3 and Bt IPS 78 expressed the lowest and the highest activities with the substrate Carboxymethyl-Chitin Remazol Brilliant Violet 5R (CM-chitin RBV 5R) respectively. When the culture supernatants of isolates grown in nutrient broth supplemented with regenerated chitin were electrophoresed on activity gels, one chitinase band of approximate molecular mass of 36 kDa was obtained from all four sub species. Future experiments can be carried out to find out whether these chitinases have any insecticidal activity aiming, to develop environmental-friendly biopesticides, and in vivo antifungal activities to test against plant pathogenic fungi in growth chambers and under glasshouse condition.

Bacillus thuringiensis chitinase 4-methylumbelliferyl substrates Carboxymethyl-Chitin Remazol Brilliant Violet 5R chitinase inducing medium

References (35)

  1. 1 Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 [DOI]
  2. 2 Plant chitinases [DOI]
  3. 3 Plant and Bacterial Chitinases Differ in Antifungal Activity [DOI]
  4. 4 Detection of chitinase activity after polyacrylamide gel electrophoresis [DOI]
  5. 5 The chitinase of Serratia marcescens [DOI]
  6. 6 Chitinases of fungi and plants: their involvement in morphogenesis and host-parasite interaction [DOI]
  7. 7 Review of Fungal Chitinases [DOI]
  8. 8 Biological control of Sclerotinia minor using a chitinolytic bacterium and actinomycetes [DOI]
  9. 9 Chitinase production by Streptomyces viridificans: its potential in fungal cell wall lysis [DOI]
  10. 10 Antifungal activity and enhancement of plant growth by Bacillus cereus grown on shellfish chitin wastes [DOI]
  11. 11 Identification of an Antifungal Chitinase from a Potential Biocontrol Agent, Bacillus cereus 28-9 [DOI]
  12. 12 Bioconversion of shellfish chitin wastes for the production of Bacillus subtilis W-118 chitinase [DOI]
  13. 13 Extracellular Chitinases of Fluorescent Pseudomonads Antifungal to Fusarium oxysporum f. sp. dianthi Causing Carnation Wilt [DOI]
  14. 14 A rapid and sensitive assay for chitinase using tritiated chitin [DOI]
  15. 15 Ethylene: Symptom, Not Signal for the Induction of Chitinase and β-1,3-Glucanase in Pea Pods by Pathogens and Elicitors [DOI]
  16. 16 Dye-labelled substrates for the assay and detection of chitinase and lysozyme activity [DOI]
  17. 17 EFFECT OF MEDIA ON GROWTH AND INTERACTIONS BETWEEN A RANGE OF SOIL‐BORNE GLASSHOUSE PATHOGENS AND ANTAGONISTIC FUNGI [DOI]
  18. 18 Antifungal activity of a novel endochitinase gene (chit36) fromTrichoderma harzianum RifaiTM [DOI]
  19. 19 A rapid and sensitive microassay for determination of chitinolytic activity [DOI]
  20. 20 Chitinolytic activity of Pseudomonas fluorescens isolates from barley and sugar beet rhizosphere [DOI]
  21. 21 Purification and characterization of an exochitinase fromBacillus thuringiensissubsp.aizawaiand its action against phytopathogenic fungi [DOI]
  22. 22 Distribution of phenotypes among Bacillus thuringiensis strains [DOI]
  23. 23 Isolation and characterization of thermostable chitinases from Bacillus licheniformis X-7u [DOI]
  24. 24 A constitutively expressed 36kDa exochitinase from Bacillus thuringiensis HD-1 [DOI]
  25. 25 Molecular characterization of a novel chitinase from Bacillus thuringiensis subsp. kurstaki [DOI]
  26. 26 Chitinase from Serratia marcescens [DOI]
  27. 27 Identification of the Substrate Interaction Region of the Chitin-Binding Domain of Streptomyces griseus Chitinase C [DOI]
  28. 28 Enhancement of the antifungal activity ofBacillus subtilisF29-3 by the chitinase encoded byBacillus circulans chiAgene [DOI]
  29. 29 Cloning of chitinase gene from Bacillus thuringiensis
  30. 30 Microbial degradation of shrimp‐shell waste [DOI]
  31. 31 Antifungal activity of a novel endochitinase gene (chit36) from Trichoderma harzianum Rifai TM [DOI]
  32. 32 L-form bacteria of Pseudomonas syringae pv. phaseolicola induce chitinases and enhance resistance to Botrytis cinerea infection in Chinese cabbage [DOI]
  33. 33 Chitinolytic activity of Pseudomonas fluorescens isolates from barley and sugar beet rhizosphere [DOI]
  34. 34 Purification, characterization and cloning of a chitinase from Bacillus sp. NCTU2 [DOI]
  35. 35 Role of chitinases in bacterial insect pathogens.

Cited by 2

Molecular characterization of the chitinase genes of native Bacillus thuringiensis isolates and their antagonistic activity against three important phytopathogenic fungi

Zehra Busra Azizoglu, Semih Yilmaz, Ugur Azizoglu · Biologia · 2021

Showing 1 of 2 known citations — external sources report more than can currently be individually listed.

Related research

Article metrics

Real usage data collected on this platform.

29

Page views

0

PDF downloads

4

Outbound clicks

2

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.