In vitro Multiple Shoot Induction and Plant Regeneration from Single-node Cuttings of Jasminum azoricum L.

Abstract

The present investigation was undertaken to induce multiple shoot from single-node cuttings of Jasminum azoricum L. (Mysore Mallige) during the year 2022-23. Single-node cuttings derived from young shoots were cultured on half-strength Murashige and Skoog (MS) medium supplemented with varying concentrations and combinations of 6-benzylaminopurine (BAP: 0.0–2.5 mg/L), kinetin (Kin: 0.0–2.5 mg/L) and adenine sulphate (Ads: 50 mg/L) for shoot induction. The nutrient medium was incorporated with different concentrations and combination of Cytokinins were used. Among all treatment combinations, the earliest shoot induction (7.16 days) and maximum shoot length (6.33 cm) were observed on medium containing BAP 1.5 mg/L + Kin 2.5 mg/L + Ads 50 mg/L. The highest number of shoots per explant (3.00) was recorded in the treatment containing BAP 2.5 mg/L + Kin 1.0 mg/L + Ads 50 mg/L. For rooting, among various concentrations of indole-3-butyric acid (IBA: 1.0–2.5 mg/L) and indole-3-acetic acid (IAA: 1.0–2.5 mg/L) used, IBA 2.5 mg/L resulted in the early root initiation (8.33 days), the highest number of roots per shoot (3.16), and the maximum root length (6.56 cm) at 45 days, whereas no significant response was observed with IAA treatments. The established protocol can be utilized for large-scale rapid multiplication to fulfil the demand of quality planting material of Mysore mallige (Jasminum azoricum L.)