In-Vitro Antioxidant, Anti-Arthritis, Anti-inflammatory, Thrombolysis, Anti-bacterial, and in-Vivo Neuropharmacological Activities of Bioactive Metabolites of Solanum americanum Mill.

Abstract

Aims: The purpose of this research was to examine the effects of methanol-prepared leaf extracts from the Solanum americanum Mill. plant on a variety of in vitro activities, including antioxidant, anti-Arthritis, anti-inflammatory, thrombolysis, anti-bacterial, and in-vivo neuropharmacological activities. Study Design: Methanolic extract of Solanum americanum leaf (MESAL) was primarily evaluated via phytochemical screening. The potential for in vitro activities, including antioxidant, anti-arthritis, anti-inflammatory, thrombolysis, and anti-bacterial activity were determined to study due to the pharmacological interest in the plant's chemical constituent. Whether there is statistical significance to the changes seen in its in vivo neuropharmacological characteristics when tested in experimental animals. Place and Duration of Study: This research was carried out between November 2022 to January 2023 at the Laboratory of Phytochemistry and Pharmacology in the Department of Pharmacy and Laboratory of Microbiology, Stamford University Bangladesh and Bangladesh Council of Scientific and Industrial Research, Dhaka. Methodology: The plant was subjected to phytochemical screening utilizing a variety of test reagents and potential antioxidant, anti-arthritis, anti-inflammatory, thrombolytic, anti-bacterial, and neuropharmacological activities of MESAL were investigated.  Antioxidant, anti-arthritic, anti-inflammatory, thrombolytic, and antibacterial activities were tested at different doses utilizing the DPPH Free Radical Scavenging Assay, Protein Denaturation Assays, Clot Lysis, and the Disk Diffusion Method. Swiss albino mice were tested using open-field and hole-cross methods to measure their locomotion as part of the neuropharmacological study. Results: The MESAL phytochemical screening findings demonstrated that the plants' chemical compositions varied. Most antioxidant activities were found in MESA, with an IC50 of 11.73 µg/mL compared to Ascorbic acid's IC50 of 28.86 µg/mL. When compared to the standard, the percent inhibition value of MESAL's anti-arthritis activity was significantly higher. With a maximum protein denaturation value of 94.03% at a concentration of 1000µg/mL, MESAL possesses potent anti-inflammatory activity. The value of MESAL in terms of clot lysis is very significant which is 90.257%. While MESAL's antibacterial value is moderate, it is still worth considering. However, the extract was proven to be less effective than Diazepam in improving motor coordination in the Open Field and Hole Cross Tests. Conclusion: Several pathological conditions, including neurodegenerative diseases, may benefit in the future from the use of plant-derived pharmacological agents due to their neuropharmacological, antioxidant, anti-inflammatory, and anti-arthritic activity, which can replace the use of NSAIDs.