In vitro Anti-inflammatory Properties of Various Extracts of Andrographis echioides

Abstract

Background: Andrographis echioides (Acanthaceae) which is commonly known as false water willow is a herb commonly found throughout India. Some of them are medicinally important. The plants from genus Andrographis are used in goiter, liver diseases, fever, fertility problems, bacterial, malarial, helminthic fungal, diarrhea and larvicidal disorders. Materials and Methods: The in-vitro anti inflammatory properties of aqueous, ethanolic, chloroform extracts were evaluated by Protein denaturation assay. Aspirin was a positive control. The experiment was done in triplicates and percent inhibition of protein denaturation was calculated. The data were analysed statistically using one way analysis of variance ( ONE-WAY ANOVA). Duncan's multiple tests were used to analyse the statistical significance between groups. The level of significance was considered at the levels of p<0.005. Results: There was a dose-dependent increase in protease inhibition of protein denaturation in three extracts from the concentration ranging from 100 to 500 ug/ml. All the three extracts of Andrographis echioides showed better in-vitro anti-inflammatory effect compounds. Conclusion: The study concluded an innovative finding that aqueous, ethanolic, chloroform of Andrographis echioides possessed potent in vitro anti inflammatory effect attributed to its flavonoid, phenyl glycosides compounds.