In-vitro and In-silico Anti-inflammatory Activity of a Common Bangladeshi Fruit: An Approach to Find Selective COX-2 Inhibitors
Md. Rabiul Hossain, Syeda Ridita Sharif, Md. Abdul Motaleb Bhuiya, Md. Mahmudul Hasan, Shankar Sharma
European Journal of Medicinal Plants · pp. 373–383 · Published 28 Dec 2024
10.9734/ejmp/2024/v35i61233Abstract
Background: From the ancient time, medicinal plants are utilized to mitigate different disease. In Ayurveda and Siddha indigenous medicinal systems, the bark of Syzygium cumini (L.) (Myrtaceae) is used for sore bronchitis, asthma, throat, dysentery, seasickness and ulcers. Objectives: The present experiment was executed to explore the anti-inflammatory property of Syzygium cumini fruit from it’s methanolic extract through phytochemical screening as well as the in-silico approach. Methods: In this experiment, standard method such as human red blood cell (HRBC) membrane stabilizing method was utilized to discover the phytochemical responsible for anti-inflammatory activity present in methanolic crude extract of Syzygium cumini fruits. In-silico ADME/T and molecular docking study was performed to assess the potential of stated phytochemicals against Cyclooxygenase-II enzyme. Findings: The presence of flavonoids, alkaloids, glycosides, tannins and saponins were confirmed by phytochemical screening. In membrane stabilizing method, the extract showed a maximum membrane stabilizing effect on HRBC with 62.381% at 1000 µg/mL in hypotonic solution 37.619% hemolysis were found when compared with standard drug Diclofenac sodium exhibited 87.143% protection. Twenty-three phytochemicals exhibit notable pharmacokinetics properties and passed drug likeness screening test in silico. In molecular docking study, Dihydromyricetin showed docking score (-9.1 kJ/mol) near as high as standard drug Celecoxib (-9.5 kJ/mol) with significant non-bonding interactions with target enzyme. Conclusions: The result of the present study demonstrates a favorable baseline in progression for the possible use of fruit of Syzygium cumini to treat inflammation.
References (28)
- 1 The Protein Data Bank [DOI]
- 2 SwissADME: a free web tool to evaluate pharmacokinetics, drug-likeness and medicinal chemistry friendliness of small molecules [DOI]
- 3 Experimental and computational approaches to estimate solubility and permeability in drug discovery and development settings [DOI]
- 4 Molecular Properties That Influence the Oral Bioavailability of Drug Candidates [DOI]
- 5 Bulletin of the World Health Organization
- 6 Origin and physiological roles of inflammation [DOI]
- 7 Experimental and computational approaches to estimate solubility and permeability in drug discovery and development settings [DOI]
- 8 Fast Calculation of Molecular Polar Surface Area as a Sum of Fragment-Based Contributions and Its Application to the Prediction of Drug Transport Properties [DOI]
- 9 Structural basis for selective inhibition of cyclooxygenase-2 by anti-inflammatory agents [DOI]
- 10 Medicinal plants in therapy [DOI]
- 11 Structure-Based Virtual Screening for Drug Discovery: Principles, Applications and Recent Advances [DOI]
- 12 Protein–ligand docking: Current status and future challenges [DOI]
- 13 Syzygium cumini (L.) Skeels: A review of its phytochemical constituents and traditional uses [DOI]
- 14 Membrane stabilization as a mechanism of the anti-inflammatory activity of methanol extract of garden egg (Solanum aethiopicum) [DOI]
- 15 Inflammaging and cardiovascular disease: Management by medicinal plants [DOI]
- 16 Differentiation of Monocytoid THP-1 Cells with Phorbol Ester Induces Expression of Prostaglandin Endoperoxide Synthase-1 (COX-1) [DOI]
- 17 Ethnopharmacology and western medicine [DOI]
- 18 COX-2 Specific Inhibitors Offer Improved Advantages Over Traditional NSAIDs [DOI]
- 19 Herbal medicine : Syzygium cumini :A Review
- 20 Applications of Docking and Molecular Dynamic Studies on the Search for New Drugs Against the Biological Warfare Agents Bacillus anthracis and Yersinia pestis [DOI]
- 21 GC-MS Analysis and Antimicrobial Assessment of Syzygium cumini (L.) Skeels Seed Ethanol Extract [DOI]
- 22 Documenting and evaluating herbal remedies [DOI]
- 23 Cyclooxygenase isoenzymes and newer therapeutic potential for selective COX-2 inhibitors [DOI]
- 24 Anti-inflammatory activity of medicinal plants native to Bangladesh: A review
- 25 Topical anti-inflammatory potential of pumpkin (Cucurbita pepo L.) seed oil on acute and chronic skin inflammation in mice
- 26 In-silico Molecular Docking Studies of some Isolated Phytochemicals from Biophytum veldkampii against Cyclooxygenase-II Enzyme and in vivo Anti-inflammatory Activity [DOI]
- 27 Current Topics in Medicinal Chemistry [DOI]
- 28 International Journal of Pharma Sciences and Research [DOI]
Cited by 6
F. Ripa, Ajoy Paul Adar, Adwiza Chakraborty Bishakha · BioMed Research International · 2026
Yalini Sadasivam, V. Pinfield, A. Trybala · Molecules · 2025
Showing 2 of 6 known citations — external sources report more than can currently be individually listed.
Related research
- A Randomized Placebo Controlled Study to Determine the Efficacy of Oral Diclofenac Sodium in the Treatment of Symptomatic Osteoarthritis of the Knee in Nnewi, South Eastern Nigeria — shares topic coverage
- Evaluation of Cytotoxicity of Diclofenac Sodium on L929 Fibroblasts - An in vitro Study — shares topic coverage
- The Potential Therapeutic Benefit of Diclofenac Sodium in Treatment of Patients with Type-2 Diabetes Mellitus — shares topic coverage
- Formulation and Evaluation of Eudragit Rl100 Polymeric Drug Loaded Microsponge for Ophthalmic Use — shares topic coverage
- Interpolymer Complex of Chitosan and Eudragit L-100: Preparation, Characterization and Drug Release Behavior — shares topic coverage
Article metrics
Real usage data collected on this platform.
57
Page views
0
PDF downloads
15
Outbound clicks
6
Citations
Views over time
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
Traffic sources
Referring site, by host.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.