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Research Article Open access CC BY 4.0

Improvement of Safflower (Carthamus tinctorius L.) for Salinity Tolerance under in vitro Condition

S. D. Surbhaiyya, D. R. Dhumale, M. S. Dudhare, P. V. Jadhav, M. P. Moharil

International Journal of Environment and Climate Change · pp. 1978–1989 · Published 20 Jun 2023

10.9734/ijecc/2023/v13i82155

Abstract

Aim: The primary aim of the present study was to screen the salt tolerant calli and optimization of   in-vitro regeneration protocol from selected screen calli. Methodology: The cotyledonary leaf explants was sterilized by using 1% Bavistin, 0.1% Mercuric chloride and 70% ethanol followed by washing with distilled water. Sterilized explants were cultured on Murashige and Skoog (MS) medium supplemented with different concentration of NaCl (i.e. 0, 50mM, 100mM, 200mM, 300 mM and 400 mM) to check the salinity tolerance ability of PKV pink genotype. In vitro screening of callus was studied by morphological characters like colour and texture of callus, callus growth percentage, relative water contained, cell survivility and proline content from saline stress and unstress calli to confirm the saline tolerance pressure to regenerate the PKV Pink safflower genotype. Results: MS medium supplemented with 150 mM NaCl showed 50% survival of calli, whereas no growth was obtained in high concentration of NaCl. Moreover, biochemical assay like proline estimation was done for its confirmation from different NaCl stress and unstress calli. The proline accumulation found to be highest callus grown on MS media supplemented with 150 mM NaCl as compared to control. Also studied the morphological observation i.e. colour and texture of calli, callus growth percentage, relative water content and cell viability under different NaCl stress to select the saline tolerance pressure to regenerate the tolerance line in PKV Pink. The saline tolerance shoots failed to produce in vitro rooting on the standardized rooting medium. So, the different approach like higher auxin shock and grafting experiment were attempted to overcome the rooting problem. Higher auxin shock experiment failed but grafting approach found satisfactory to overcome the rooting in saline tolerance shoot and for development of saline tolerance line in PKV Pink safflower genotype. Conclusion: The development of abiotic stress tolerance plants found the better understanding of physiological and biochemical changes in plants under in vitro stress conditions. 

Safflower regeneration salinity tolerance screening

References (22)

  1. 1 Sodium (Na+) homeostasis and salt tolerance of plants [DOI]
  2. 2 Putrescine alleviation of growth in salt stressed Brassica juncea by inducing antioxidative defense system [DOI]
  3. 3 Developing stress tolerant plants through in vitro selection—An overview of the recent progress [DOI]
  4. 4 Crop breeding for salt tolerance in the era of molecular markers and marker‐assisted selection [DOI]
  5. 5 Salinity and water stress have contrasting effects on the relationship between growth and cell viability during and after stress exposure in durum wheat callus [DOI]
  6. 6 Micropropagation of Withania somnifera from germinating seeds and shoot tips [DOI]
  7. 7 The effect of salt stress and abscisic acid on proline production, chlorophyll content and growth of in vitro propagated shoots of Eucalyptus camaldulensis [DOI]
  8. 8 Effects of NaCl and mannitol induced stress on sugarcane (Saccharum sp.) callus cultures [DOI]
  9. 9 Growth, proline and ion accumulation in sugarcane callus cultures under drought-induced osmotic stress and its subsequent relief [DOI]
  10. 10 Agrobacterium-mediated transformation of safflower and the efficient recovery of transgenic plants via grafting [DOI]
  11. 11 Central Role of Salicylic Acid in Resistance of Wheat Against Fusarium graminearum [DOI]
  12. 12 Biochemical characterization and identification of differentially expressed candidate genes in salt stressed sugarcane [DOI]
  13. 13 TDZ x IBA induced shoot regeneration from cotyledonary leaves and in vitro multiplication in safflower ( Carthamus tinctorius L.) [DOI]
  14. 14 Impact of salt stress on photosystem II efficiency and antioxidant enzyme activities of safflower (Carthamus tinctorius L.) cultivars [DOI]
  15. 15 Exogenous application of penconazole regulates plant growth and antioxidative responses in salt-stressed Mentha pulegium L. [DOI]
  16. 16 Breeding improvements in safflower ('Carthamus tinctorius' L.): A review
  17. 17 Role of proline and glycinebetaine in improving chilling stress tolerance in sugarcane buds at sprouting
  18. 18 Effects of saline and mannitol induced stress on some biochemical and physiological parameters of Carthamus tinctorius L. varieties callus cultures
  19. 19 Improvements in rooting regenerated safflower (Carthamus tinctorius L.) shoots [DOI]
  20. 20 Effects of drought stress on biochemical and physiological parameters in callus cultures of Carthamus tinctorius varieties [DOI]
  21. 21 In Vitro Selection of Foeniculum vulgare for Salt Tolerance [DOI]
  22. 22 International Journal of Advanced Biological and Biomedical Research [DOI]

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