Identification of SSR Markers Linked to Mung Bean Yellow Mosaic Virus Resistance in Blackgram (Vigna mungo (L.) Hepper) Using Bulked Segregant Analysis in F2:3 Population

Abstract

Aims: In blackgram (Vigna mungo (L.) Hepper), mung bean mung bean yellow mosaic virus (MYMV) disease causes severe yield reduction. MAS (Marker Assisted Selection) can be used to improve the selection efficiency for MYMV resistance. Hence the present study was carried out to use bulked segregant analysis (BSA) and simple sequence repeat (SSR) marker validation tests to find the simple sequence repeat (SSR) markers associated to MYMV resistance in the blackgram segregating population, (F2:3) of ADT 3 x IC343856. Study Design: F2:3 generated 60 single plants of ADT 3 x IC343856 were raised with regular rows of MYMV susceptible check variety CO 5 to draw white flies and thus to improve MYMV infection under field screening. To identify the SSR markers associated to MYMV resistance, bulk segregant analysis and marker validation tests were conducted. Place and Duration of Study: The experiment was carried out at Department of Genetics and Plant Breeding, VOC Agricultural College and Research Institute, Killikulam, Tamil Nadu Agricultural University, India from 2020 to 2022. Methodology: The F2:3 mapping population of the cross, ADT 3 x IC 343856 was used for BSA using 50 simple sequence repeat (SSR) markers for mung bean mung bean yellow mosaic virus (MYMV) resistance studies in blackgram. Results: Four markers viz., CEDG008, CEDG271, VM6 and CEDG264 exhibited polymorphism between the parents. The F2:3 segregants were raised along with their parents and check variety CO 5 was raised as infector rows to ensure the disease incidence in the population. An equal number of two extreme genotypes (10 resistant and 10 susceptible respectively) were pooled to form the bulks. Among the four polymorphic markers studied, CEDG 008 was able to differentiate resistant and susceptible bulks and their corresponding individuals (120 bp and 110 bp respectively). From the previous reports, it has been confirmed that CEDG 008 is a potential marker for MYMV resistance studies in different genetic backgrounds.