Molecular Typing and Antimicrobial Susceptibility of Acinetobacter baumannii Isolates in Kermanshah City with Pulse Field Gel Electrophoresis (PFGE)

Abstract

Background and Objective: Acinetobacter baumannii (A. baumannii) in the last decade has been identified as one of the opportunistic pathogens and an important cause of nosocomial infections. Molecular typing plays an important role in studying the epidemiology of Acinetobacter. The aim of this study was to investigate the genomic pattern which was performed by Pulse Field Gel Electrophoresis (PFGE) and antimicrobial susceptibility of the isolates from patients in various hospitals in the city of Kermanshah, the city which is located in the west of Iran. Materials and Methods: 33 isolates of A. baumannii were collected from clinical samples in four general hospitals of Kermanshah. Isolates were identified by biochemical tests and API 20NE kit. The antimicrobial susceptibility of isolates was determined by Kirby-Bauer disk diffusion method. The clonal connection was estimated by PFGE  and DNA patterns were analyzed by Gel compare II 6.5 software. Results: All isolates showed high-level of resistance, but resistance which was observed against Colistin and Minocycline was low, while no resistance to Polymyxin B and Tigecycline was observed. The PFGE analysis revealed the existence of 10 different genetic patterns among the 33 strains including: I (n = 12), II (n = 2), III (n = 4), IV (n = 3), V (n = 3), VI (n = 4), VII (n = 2). Clone I was the dominant clone. In terms of antibiotic resistance, no significant difference was observed among the different genetic patterns. Conclusion: Isolates were obtained from a large variety of patterns genetically. This study could represent the wide range of isolates of A. baumannii that were gathered from different parts of the hospital. Diverse sources of infection may, therefore, appear to control these infections, according to various sources, which are not simple.