Poulk Plant (Stachys schtschegleevii) and Its Antibacterial Specifications

Abstract

Poulk plant (Stachys schtschegleevii Sosn. ex Grossh.) is one of the medicinal plants with a long history in traditional medicine and is used to remedy many diseases. Polk plant possesses some properties, including the internal anti-infectious, antibacterial, Anti-asthma, anti-sinusitis, anti-inflammatory and it is used to remedy the respiratory inflammatory diseases and has been identified asa natural penicillin. This study aims to investigate the phytochemical and antibacterial effects of ethanolic extract, 2-Propanol and n-hexane of the Poulk plant against Escherichia coli and Staphylococcus aureus, Bacillus subtilis and Salmonella enterica. In this study, extracting was done by Soxhlet extractor with ethanolic, 2-Propanol and n-hexane solvents and after evaporation of the solvent and methylation by the rotary evaporator, the obtained substance was injected into the GC-MASS and the substance was detected, as well as the Inhibition zone, Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) studies were carried out. The ranges of MIC and MBC of ethanolic extract, 2-Propanol and n-hexane for MIC were 0.78-12.5%, 0.78 -6.25% and 12.5-50%, respectively. MBC for ethanolic extract, 2-Propanol and n-Hexane were 0.78-12.5%, 0.78 -6.25% and 12.5-100%, respectively.