Phytochemical Screening and Antibacterial Evaluation of Randia acuminata Root Extract against Wound Pathogens
Ofonime U. M. John, Anthony N. Umo, Samuel I. Eduok
Asian Journal of Biology · pp. 1–9 · Published 15 Jun 2018
10.9734/AJOB/2018/41701Abstract
Aims: The aim of the present study was to determine common pathogens associated with infected wounds and the phytochemical composition and antibacterial activities of Randia acuminata root extract against isolates associated with infected wounds. Study Design: The ethanolic extract was used for the phytochemical screening of the root extract. The disc diffusion method was used to assess the antibacterial activities of the alkaloid fraction of the root extract against isolated pathogens. Place and Duration of Study: The study was carried out at the University of Uyo Teaching Hospital (Microbiology Laboratory) and Department of Microbiology (Microbiology Laboratory), University of Uyo, Uyo, Akwa Ibom State, Nigeria between the months of April, 2017 – August, 2017. Methodology: The isolation of infective agents from wound specimens and the phytochemical screening of the root extract were performed using standard methods. The disc diffusion method was used to evaluate the antibacterial activities of the alkaloidal fraction of the root extract on Staphylococcus aureus and species of Streptococcus, Pseudomonas, Klebsiella and Clostridium. Results: Alkaloid, saponins, phlabotanin, anthraquinone deoxysugar, cardiac glycoside and terpenes constitute the bioactive components of the root extract. The pathogens associated with infected wounds and their frequency of occurrence were Staphylococcus aureus (35%) and species of Streptococcus (15%), Pseudomonas (20%) Klebsiella (20%) and Clostridium (10%). Susceptibility of the organisms to the root extract varied with concentration 30 < 40 < 50mg/ml The antibacterial activity of the extract suggests concentration-dependent inhibitory response by the test organisms. Conclusion: The results of this study indicate that the alkaloid fractions of R. acuminata root extract has potent antibacterial effect and can be incorporated into antiseptics for wound cleaning/dressing.
Cited by 1
Alejandro Martínez-Ceja, Antonio Romero-Estrada, María Crystal Columba-Palomares · South African Journal of Botany · 2022
Related research
- Antibacterial Activities of Streptomyces collinus and Streptomyces refuineus from Cocoa, Orange and Timber Plantation Soils in Akure Metropolis — shares topic coverage
- Antimicrobial Activity, Phytochemical Screening and Nutrient Analysis of Tetrapleura tetraptera and Piper guineense — shares topic coverage
- Antibacterial and Antibiofilm Properties of the Crude Ethanolic, Methanolic and Aqueous Bark and Seed Extracts of Citrullus lanatus Fruit — shares topic coverage
- Susceptibility of Some Clinically Resistant Bacterial Isolates to Thyme Essential Oil, Chitosan and Lactobacillus reuteri — shares topic coverage
- Antibacterial and Antifungal Effect of Cinnamon — shares topic coverage
Article metrics
Real usage data collected on this platform.
0
Page views
0
PDF downloads
0
Outbound clicks
1
Citations
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
No views recorded yet.
Traffic sources
Referring site, by host.
No traffic recorded yet.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.