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Research Article Open access CC BY 4.0

Hepatoprotective Activity of Nigella sativa Oil against Antitubercular Drug-induced Hepatotoxicity in Rats

Shazia Wahid, Parvaze Ahmad Wani, Nazia Wahid, Neelofar Jan

Journal of Advances in Medical and Pharmaceutical Sciences · pp. 1–8 · Published 28 Nov 2016

10.9734/JAMPS/2016/28571

Abstract

Aim: Drug induced hepatotoxicity is a potentially serious adverse effect of the currently used antitubercular chemotherapeutic regimens containing isoniazid (INH) and rifampicin. The aim of this study is to check the hepatoprotective action of Nigella sativa against the antitubercular drugs (Rifampicin and Isoniazed)-induced hepatotoxicity in rats. Place and Duration of Study: This study was carried out in the Department of Pharmacy, Integral University, Lucknow, UP, India in the year 2011. Methodology: Nigella sativa oil was purchased from local market of Lucknow India with assured quality in the month of January, 2011 and study was performed in same year.  Powder form of drugs like Rifampicin and Isoniazid were gifted by Cadila Pharmaceuticals limited, Ahmedabad, Gujarat, India whereas 24 Albino rats (Wistar strain) female, weighing 120–150 g, were procured from the Animal House Facility, Integral University, Lucknow. Rats were divided into four groups; first group which is an untreated control was given only standard diet. Second group was treated with Nigella sativa, which served as drug control. Third group were treated with 100 mg of each Isoniazid and Rifampicin/kg of body weight. In fourth group rats were treated with Nigella sativa oil and 100 mg of Isoniazid and Rifampicin/kg of body weight. At the end of dosing, serum was used for estimation of marker enzymes like aspartate aminotransferase (AST), lactate dehydrogenase (LDH), alanine aminotransferase (ALT), alkaline phosphatase (ALP) and total and direct bilirubin. Grading of treated and untreated liver was also done. Results: This study showed that Nigella sativa acted as antiinflammatory and antinecrotic in isoniazid and rifampicin administered drugs in rats. When Nigella sativa was coadminstered with Rifampicin + Isoniazid, it resulted in the decrease of these marker enzymes and maintained these enzymes at normal levels in the serum of rats compared to the only Rifampicin + Isoniazid administered rats thus indicates the hepatoprotective action of Nigella sativa. Conclusions: Due to above properties of Nigella sativa can be used as hepatoprotective against antitubercular drugs.  

Nigella sativa hepatoprotective antioxidant activity rifampicin isoniazid grading of liver rats

References (16)

  1. 1 An Algorithm for the Grading of Activity in Chronic Hepatitis C [DOI]
  2. 2 Antioxidant activity of Nigella sativa essential oil [DOI]
  3. 3 Estimation of Plasma Phosphatase by Determination of Hydrolysed Phenol with Amino-antipyrine [DOI]
  4. 4 Fixed Oil ofNigella sativaand Derived Thymoquinone Inhibit Eicosanoid Generation in Leukocytes and Membrane Lipid Peroxidation [DOI]
  5. 5 Oxidative damage and defense [DOI]
  6. 6 The effect of Nigella sativa oil against the liver damage induced by Schistosoma mansoni infection in mice [DOI]
  7. 7 Protective effect of cysteine and vitamin E, Crocus sativus and Nigella sativa extracts on cisplatin-induced toxicity in rats.
  8. 8 Protective effects of thymoquinone and desferrioxamine against hepatotoxicity of carbon tetrachloride in mice [DOI]
  9. 9 Mucoadhesive chitosan-coated liposomes: characteristics and stability [DOI]
  10. 10 Nigella sativa L. Oil Protects against Induced Hepatotoxicity and Improves Serum Lipid Profile in Rats [DOI]
  11. 11 Oxidative burst of Kupffer cells: target for liver injury treatment. [DOI]
  12. 12 The protective effects of ursodeoxycholic acid on isoniazid plus rifampicin induced liver injury in mice [DOI]
  13. 13 Hepatotoxicity caused by the combined action of isoniazid and rifampicin. [DOI]
  14. 14 Protective effect of Indian honey on acetaminophen induced oxidative stress and liver toxicity in rat [DOI]
  15. 15 Hepatoprotective effect of Anacyclus pyrethrum Linn. against antitubercular drug-induced hepatotoxicity in SD rats [DOI]
  16. 16 Basic Physiology and Anatomy [DOI]

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