Genetic Diversity Assessment in Chickpea Using Morphological Characters and Start Codon Targeted (SCoT) Molecular Markers
Kamal Mohammad-Said Ahmad, Reza Talebi
Annual Research & Review in Biology · pp. 1–8 · Published 28 Jul 2017
10.9734/ARRB/2017/34203Abstract
Genetic diversity in 35 chickpea genotypes were studied using morphological characters and 14 start codon targeted (SCoT) markers. Results of variance analysis and descriptive statistics for morphological traits indicated that the genotypes differed significantly for all studied characteristics. A dendrogram was constructed from morphological traits and the genotypes were grouped into six clusters. Fourteen SCoT primers yielded 135 bands, of which 100 bands were polymorphic. Number of polymorphic bands varied from 6 to 9, with an average of 7.14 bands per primer. PIC values ranged from 0.27 (SCoT22) to 0.46 (SCoT15), with an average value of 0.36 per primer. Cluster analysis Based on SCoT-PCR markers grouped 35 chickpea genotypes into three major clusters. Results showed a weak relationship between morphological divergence and molecular diversity pattern. Overall, we found relatively high genetic diversity in examined chickpea genotypes using morphological and SCoT molecular markers. Findings of this study can be useful for breeder for selective genotypes and specific traits in breeding programs in chickpea.
References (15)
- 1 Analysis of Gene Diversity in Subdivided Populations [DOI]
- 2 Start Codon Targeted (SCoT) Polymorphism: A Simple, Novel DNA Marker Technique for Generating Gene-Targeted Markers in Plants [DOI]
- 3 Data Analysis Methods [DOI]
- 4 iPBS: a universal method for DNA fingerprinting and retrotransposon isolation [DOI]
- 5 Characterization of genetic diversity in chickpea using SSR markers, Start Codon Targeted Polymorphism (SCoT) and Conserved DNA-Derived Polymorphism (CDDP) [DOI]
- 6 Genetic diversity in major farm crops on the farm and in reserve [DOI]
- 7 The extent of clonality and genetic diversity in lingonberry (Vaccinium vitis‐idaea L.) revealed by RAPDs and leaf‐shape analysis [DOI]
- 8 Relationships among different geographical groups, agro-morphology, fatty acid composition and RAPD marker diversity in Safflower (Carthamus tinctorius) [DOI]
- 9 Comparison of genetic variation and differentiation among annual Cicer species using start codon targeted (SCoT) polymorphism, DAMD-PCR, and ISSR markers [DOI]
- 10 Genetic Diversity of Iranian Accessions, Improved Lines of Chickpea (Cicer arietinum L.) and Their Wild Relatives by Using Simple Sequence Repeats [DOI]
- 11 Variation in the Agronomic and Morphological Traits of Iranian Chickpea Accessions [DOI]
- 12 Geographical patterns of genetic diversity in cultivated chickpea (Cicer arietinum L.) characterized by amplified fragment length polymorphism [DOI]
- 13 Geographical patterns of variation for morphological and agronomic characteristics in the chickpea germplasm collection [DOI]
- 14 Exploration of biochemical and molecular diversity in chickpea seeds to categorize cold stress-tolerant and susceptible genotypes [DOI]
- 15 Use of RAPD Markers in Comparison with Agro-morphological Traits for Estimation of Diversity among Chickpea Genotypes [DOI]
Cited by 3
Z. Hromadová, Lucia Mikolášová, Ž. Balážová · Journal of Microbiology, Biotechnology and Food Sciences · 2022
B. Hussein, N. Osman · 2020
M. Z. Ahmed, I. M. Masoud, S. Zedan · 2019
Related research
- Genetic Diversity Study of Locally Isolated Bacillus thuringiensis Strains from Kuwait Using Random Amplified Polymorphic DNA Analysis — shares topic coverage
- Genetic Diversity and Distribution of Cassava Brown Streak Virus and Ugandan Cassava Brown Streak Virus in Major Cassava-growing Regions in Kenya — shares topic coverage
- Species-specific Loci of Three Indonesian Durio Inferred from ISSR Fingerprinting — shares topic coverage
- Diversified Strains of Porcine Reproductive and Respiratory Syndrome Virus Circulating in China: A Mini-overview — shares topic coverage
- Genetic Characterisation of Honey Bees (Apis mellifera) Populations from Kurdistan Region of Iraq via ISSR Markers — shares topic coverage
Article metrics
Real usage data collected on this platform.
52
Page views
0
PDF downloads
28
Outbound clicks
3
Citations
Views over time
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
Traffic sources
Referring site, by host.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.