Influence of Different Auxins on Rooting of Rosemary, Sage and Elderberry

Abstract

Aims: The aim of research is to examine the influence of auxins IAA, IBA, NAA and commercial rooting product on rooting of cuttings from rosemary (Rosmarinus officinialis L.), sage (Salvia officinialis L.) and elderberry (Sambucus nigra L). Study Design: Completely randomized design. Place and Duration of Study: The experiment was conducted in a plastic tunnel with three beds in Stip, Republic of Macedonia, between October 2012 and January 2013. Methodology: Fifty cuttings from each experimental species (Rosmarinus officinialis L., Salvia officinialis and Sambucus nigra L.), with height of 15 cm, were treated with freshly made solutions of different auxin and planted for rooting in the rooting beds. After 100 days of planting we measure: height of cuttings, number of rooted cuttings, number of roots, root length and rooting percentage. Results: The conducted research shows that growth regulators applied during the experiment, including the commercial rooting product, positively influenced rooting process of vegetative cuttings of rosemary and sage. Application of auxins as IAA, IBA and NAA in concentration of only 5 ppm have stimulating effect on rooting of the rosemary (IBA – 92%), sage (IBA – 54%; NAA – 50%) and elderberry cuttings (K1 and IAA – 14%). Conclusion: This study is first report for application of auxins on rosemary, sage and elderberry cuttings collected from local populations in Republic of Macedonia. Application of auxins in concentrations of 5 ppm positively affected rosemary, sage and elderberry. The results presented in this paper show that the vegetative propagation of medicinal and aromatic species might be stimulated by auxins utilization even in very low concentrations. It should be noted that further research should be extended to more medicinal and aromatic plants with significant economic importance as well as with application of several higher concentration of auxins.