Tumoricidal Effect of Trigonella foenum-graceum Extract and Selenium Nanoparticles on Ehrlich Carcinoma Bearing Mice

Abstract

Trigonella foenum-graceum extract either alone or combined with selenium nanoparticles exhibited antitumor effect. Ehrlich ascite carcinoma (EAC) cell line and four groups of female mice were used. Solid Ehrlich carcinoma (EC) was induced by inoculation of 2.5x106 cells in left thighs of each animal. Mice were gavage orally by 2.5 µg/0.1 ml of Trigonella foenum-graceum extract either alone or combined with selenium nanoparticles daily during one month. Tumor size, serum tumor markers (TNF-α, IFN- γ, Granzyme-B and Caspase-3) were measured. Oxidative stress, antioxidant markers, Histopathological, apoptotic and necrotic examinations were determined in tumor tissues. Tumor size of experimental groups represents reduction. Caspase-3 as well as Granzyme-B activities were significantly elevated along with diminishing tumor size while, TNF-α and IFN-γ levels were decreased in serum. Meanwhile, oxidative stress marker (MDA) was significantly decreased in tumor tissue. The tumor GSH content and CAT activity were increased. Histopathological, apoptotic and necrotic examinations were context with previous conclusion. It could be concluded that Trigonella foenum-graceum extract either alone or combined with SeNPs exhibited antitumor effect which is reflected by a inhibition in tumor size, a decrease of serum TNF-α and IFN-γ, an increase in serum caspase-3 and Granzyme-B, reduction in tumor MDA and an increase in tumor GSH and CAT which cause regulate tumor regression.