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Research Article Open access CC BY 3.0

Frequency of Two Genetic Polymorphisms of CYP1A2 Gene in Iranian Population

Maryam Sadat Samimifar, Ali Ramazani

Annual Research & Review in Biology · pp. 1769–1776 · Published 12 Feb 2014

10.9734/ARRB/2014/7516

Abstract

Aim: The present study was conducted to explore the allelic and genotypic frequencies of CYP1A2 gene polymorphisms, CYP1A2*1C (−3860 G>A) and CYP1A2*1F (−163 C>A), among 200 healthy Iranian volunteers. Study Design: Completely randomized design. Place and Duration of Study: Place – Biotechnology Department, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran. Duration – June, 2011 to May, 2013. Methodology: DNA was extracted from the blood samples by salting-out method. Primers and probes were designed by the Primer Express® Software v3.0.1. Allelic discrimination of the CYP1A2 alleles was performed by Real-Time PCR, and the data were analyzed by the SPSS 16.00 software. Results: The frequency of each polymorphism in Iranian population was found to be 0.05 and 0.3 for CYP1A2*1C and CYP1A2*1F, respectively. Our data did not show any difference between the Iranian, Turkish, and Egyptian populations in CYP1A2*1C polymorphism; also, CYP1A2*1F polymorphism analysis did not show any differences between Iranian, Turkish, Egyptian, Chinese, Serbian, and Japanese populations. Allele frequency in our population was in equilibrium according to Hardy–Weinberg principle (P=0.05). Conclusion: This is the first study analyzing the CYP1A2 clinically important allelic variants in Iranian population. Our results indicate that functional significance of these alleles should be evaluated in various populations. It is necessary to determine the relationship between these variants and CYP1A2-related drug metabolism in associated groups.

SNP CYP1A2 real time PCR pharmacogenomics Iran

References (33)

  1. 1 A simple salting out procedure for extracting DNA from human nucleated cells [DOI]
  2. 2 Cytochrome P450 and Chemical Toxicology [DOI]
  3. 3 Polymorphism of human cytochrome P450 enzymes and its clinical impact [DOI]
  4. 4 The Cytochrome P450 Superfamily: Biochemistry, Evolution and Drug Metabolism in Humans [DOI]
  5. 5 Functional significance of a C→A polymorphism in intron 1 of the cytochrome P450 CYP1A2 gene tested with caffeine [DOI]
  6. 6 Genomic and personalized medicine: foundations and applications [DOI]
  7. 7 Coffee, CYP1A2 Genotype, and Risk of Myocardial Infarction [DOI]
  8. 8 Pharmacogenomics: The Inherited Basis for Interindividual Differences in Drug Response [DOI]
  9. 9 Cytochrome P450 variations in different ethnic populations [DOI]
  10. 10 Assessment of CYP1A2 Activity in Clinical Practice: Why, How, and When? [DOI]
  11. 11 Structure, function, regulation and polymorphism and the clinical significance of human cytochrome P450 1A2 [DOI]
  12. 12 Polymorphisms in the cytochrome P450 CYP1A2 gene (CYP1A2) in colorectal cancer patients and controls: allele frequencies, linkage disequilibrium and influence on caffeine metabolism [DOI]
  13. 13 Genome-wide association analysis of coffee drinking suggests association with CYP1A1/CYP1A2 and NRCAM [DOI]
  14. 14 Genetic Polymorphism of CYP1A2 in Ethiopians Affecting Induction and Expression: Characterization of Novel Haplotypes with Single-Nucleotide Polymorphisms in Intron 1 [DOI]
  15. 15 Human cytochrome P4501A2.
  16. 16 The TaqMan Method for SNP Genotyping [DOI]
  17. 17 Nonresponse to Clozapine and Ultrarapid CYP1A2 Activity [DOI]
  18. 18 Synthetic and Natural Compounds that Interact with Human Cytochrome P450 1A2 and Implications in Drug Development [DOI]
  19. 19 Detection of Three Genetic Polymorphisms in the 5′‐Flanking Region and Intron 1 of Human CYP1A2 in the Japanese Population [DOI]
  20. 20 Induction of CYP1A2 by heavy coffee consumption in Serbs and Swedes [DOI]
  21. 21 Coffee, CYP1A2 genotype and risk of myocardial infarction [DOI]
  22. 22 Cytochrome P450 genetic polymorphisms of Mexican indigenous populations [DOI]
  23. 23 Single Nucleotide Polymorphisms and Haplotypes of CYP1A2 in a Japanese Population [DOI]
  24. 24 The G−113A polymorphism in affects the caffeine metabolic ratio in a Chinese population [DOI]
  25. 25 Genotyping of four genetic polymorphisms in the CYP1A2 gene in the Egyptian population [DOI]
  26. 26 Techniques patents for SNP genotyping [DOI]
  27. 27 Rapid genotyping for relevant CYP1A2 alleles by pyrosequencing [DOI]
  28. 28 Genetic polymorphisms of the CYP3A4, CYP3A5, CYP3A7 and CYP1A2 among the Jordanian population [DOI]
  29. 29 Frequencies of four genetic polymorphisms in the CYP1A2 gene in Turkish population [DOI]
  30. 30 CYP1A2 polymorphism (C > A at position −163) in Ovambos, Koreans and Mongolians [DOI]
  31. 31 Techniques of SNP Genotyping and Remote Sensing Applied for Elucidation of the Contribution of Polygene and Environmental Factors to Inter-individual Variation in Skin Pigmentation Phenotype [DOI]
  32. 32 [Clinical significance of cytochrome P450 genetic polymorphism--part IV. Cytochrome P450 3A4 and 3A5].
  33. 33 European Journal of Clinical Pharmacology [DOI]

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