Persistence of Demodex Folliculorum Mites in the Eyelash Follicles of Human Cadavers

Abstract

Purpose: This study aims to investigate the presence of Demodex mites, particularly Demodex folliculorum, in the eyelash follicles of cadavers preserved through different embalming methods. The purpose is to determine the viability and/or persistence of these mites in post-mortem conditions, challenging previous assumptions about their survival limits. Methods: Four human cadavers were examined in collaboration with the Department of Ophthalmology, and eyelash samples were epilated for microscopic analysis. A modified Coston method with an olive oil solution was employed to enhance the visibility of the mites. The demographic data and preservation methods of the cadavers were recorded. Results: Microscopic examination revealed the presence of Demodex folliculorum in all cadaver samples, but no live mites were found. Skeletons of these mites were particularly evident in areas with concentrated oil residues. The mites exhibited signs of death, including the cessation of leg and mouth movements, as well as decreased autofluorescence. Conclusions: Demodex folliculorum demonstrated resilience by persisting in the eyelash follicles of cadavers preserved through different methods, even after embalming. The findings challenge existing assumptions about the survival limits of these mites and underscore the need for further research in post-mortem conditions. Additionally, the study highlights the potential risks of parasitic and/or bacterial infections for healthcare personnel working with cadavers and emphasizes the importance of awareness and preventive measures in such settings.