Experimental Use of Blood PRP for Regeneration of Manually Sciatic Nerve Damaged in Dogs

Abstract

The study has done on (10) adult healthy male dogs separated as two group (control group and group treated with PRP) each group had (5 dogs), with .body .weight ranged .between (15-20 kg). Blood was collected from peripheral cephalic vein (10 ml in anti-coagulant tube) in five dogs and double centrifugation protocol was followed at 800 rpm and 1600 rpm for 10 minutes to separate the PRP. The PRP then injected directly in the manually damaged sciatic nerve in both side of legs. The result show that the left legs showed moderate knelling, pain and swelling was also showed. The histo-pathological examination of proximal longitudinal section of sciatic nerve at 3 months after treatment showed Increase number of Schwann cell and vacuolated degenerated nerve fibers with distorted arraignment. The histo-pathological examination of middle transverse part of nerve fawned increase number of Schwann cell and vacuolated degenerated nerve fibers with distorted arraignment. The histo-pathological examination of longitudinal section of the distal part of sciatic nerve showed Irregular arrangement of nerve fibers irregular spaces in between and moderate regeneration.