Expression Study of Bmlipase in Silkworm Bombyx mori midgut  against BmNPV

Thanmaya M; Bhuvaneswari E; Mallikarjuna G; Ranjini MS; Chandrakanth N; Manjunath B; Madhusudhan KN; Shyamkumar V; Moorthy SM; Gandhi Doss S

doi:10.9734/arrb/2024/v39i112156

Research Article Open access CC BY 4.0

Expression Study of Bmlipase in Silkworm Bombyx mori midgut against BmNPV

Thanmaya M, Bhuvaneswari E, Mallikarjuna G, Ranjini MS, Chandrakanth N, Manjunath B, Madhusudhan KN, Shyamkumar V, Moorthy SM, Gandhi Doss S

Annual Research & Review in Biology · pp. 61–69 · Published 18 Nov 2024

10.9734/arrb/2024/v39i112156

Abstract

Silkworms are susceptible to various pathogens, including bacteria, fungi, viruses, and protozoa. The present study focused on the viral disease known as nucleopolyhedrosis virus, which causes severe infections in silkworms. Silkworms possess several antiviral proteins that play a crucial role in preventing the spread of infection. One protein, which is found in the digestive juice produced in the silkworm's midgut, was focused, and it is lipase. This protein is responsible for controlling the virus infection. To investigate the role of lipase expression in silkworm for virus control, its concentration after post-infection was measured. This was quantified and analysed using SDS-PAGE. The band of interest, with a molecular weight of 29 kDa, was further analysed through MALDI-TOF/MS, and it showed 70% homology to the lipase enzyme.

NPV proteins SDS-PAGE MALDI-TOF/MS

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Thanmaya M, Bhuvaneswari E, Mallikarjuna G, Ranjini MS, Chandrakanth N, Manjunath B, Madhusudhan KN, Shyamkumar V, Moorthy SM, Gandhi Doss S (2024). Expression Study of Bmlipase in Silkworm Bombyx mori midgut against BmNPV. Annual Research & Review in Biology , 61–69. https://doi.org/10.9734/arrb/2024/v39i112156

Affiliations

Thanmaya M — Central Sericultural Research and Training Institute, Mysuru, Karnataka, India.
Bhuvaneswari E — Central Sericultural Research and Training Institute, Mysuru, Karnataka, India.
Mallikarjuna G — Central Sericultural Research and Training Institute, Mysuru, Karnataka, India.
Ranjini MS — Central Sericultural Research and Training Institute, Mysuru, Karnataka, India.
Chandrakanth N — Central Sericultural Research and Training Institute, Mysuru, Karnataka, India.
Manjunath B — Department of Cell Biology and Molecular Genetics, Sri Devaraj Urs Academy of Higher Education and Research (A Deemed to be University), Kolar, Karnataka, India.
Madhusudhan KN — Central Sericultural Research and Training Institute, Mysuru, Karnataka, India.
Shyamkumar V — Department of Biotechnology and Microbiology, Karnatak University, Dharwad, Karnataka, India.
Moorthy SM — Central Silk Board, Bangalore, Karnataka, India.
Gandhi Doss S — Central Sericultural Research and Training Institute, Mysuru, Karnataka, India.

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