In-vitro Susceptibility Profile of Pseudomonas aeruginosa to Selected Medicinal Plants Obtained from University of Calabar Farm, Nigeria

Abstract

Antibiotic resistance represents the greatest challenges to global health. Strategies to curb the menace of antibiotic resistance include researching for new antimicrobials from plants. This study is aimed at determine the in-vitro susceptibility of Pseudomonas aeruginosa to Chromolaena odorata, Jatropha tanjorensis, Ocimum gratissimum, Vernonia amydalina, Hibiscus sabdariffa and Aloe barbadensis obtained within University of Calabar farm. All the test carried out were performed according to standard microbiological procedure. Ethanol was used for extraction of Phytochemicals and were purified using column chromatography. In-vitro susceptibility of selected plants against P. aeruginosa was in the order C. odorata>J. tanjorensis > O. gratissimum > H. sabdariffa >V. amydalina > A. barbadensis (Aloe vera). The result revealed that the leaves of C. odorata had the highest antimicrobial effect against P. aeruginosa. The first column of all the plant extracts showed no antimicrobial activity. MIC of C. odorata, J. tanjorensis, O. gratissimum and H. sabdariffa were observed in 10-7, 10-6, 10-5 and 10-4 dilution respectively. Statistical analysis between the zone of inhibitions and layers of column chromatography was significant (<0.05). Ethanol extract and the third column of Chromolaena odorata, Jatropha tanjorensis, Ocimum gratissimum have great potential antibacterial activity against P.  aeruginosa.