Ex-vivo Antispasmodic and In vivo  Anti Salmonella Potential of  Rumex bequaertii Leaves Extracts

Claude Ruphin Mougoue Deumeni; André Perfusion Amang; Céline Nguefeu Nkenfou; Roméo Georges Bonsou Fosin; Augustin Notedji; Roland Tchuenteu Tchuenguem; Gaël Tchokomeni Siwe; Jules-Roger Kuiate; Pierre Watcho; Paul Vernyuy Tan

doi:10.9734/ajrb/2022/v11i3-4217

Research Article Open access CC BY 4.0

Ex-vivo Antispasmodic and In vivo Anti Salmonella Potential of Rumex bequaertii Leaves Extracts

Claude Ruphin Mougoue Deumeni, André Perfusion Amang, Céline Nguefeu Nkenfou, Roméo Georges Bonsou Fosin, Augustin Notedji, Roland Tchuenteu Tchuenguem, Gaël Tchokomeni Siwe, Jules-Roger Kuiate, Pierre Watcho, Paul Vernyuy Tan

Asian Journal of Research in Biochemistry · pp. 6–14 · Published 30 Dec 2022

10.9734/ajrb/2022/v11i3-4217

Abstract

Objective: The present study aimed at investigating the acute toxicity, the ex-vivo antispasmodic potential of aqueous and ethanolic Rumex bequaertii leaves extracts in isolated ileum fragment and antimicrobial activity of Rumex bequaertii ethanolic leaves extract in animal models infected with Salmonella typhi. Methods: Acute toxicity using a single dose of ethanolic extract of Rumex bequaertii at 2000 mg/kg was administered to female rats and effects were observed during 14 days. Different cumulative concentrations of the aqueous and ethanolic extracts of Rumex bequaertii leaves were tested for spontaneous contractions and potassium chloride-induced contractions in rat ileum fragment. Different doses of the ethanolic extract of Rumex bequaertii leaves were tested for antidiarrheal activity in Wistar rats infected with Salmonella typhi. Results: Rats given the single dose of Rumex bequaertii ethanolic extract showed no significant changes in body and organs weights compared to control rats. Administration of extracts at all tested concentrations resulted to inhibition of ileum contractions. Inhibition of spontaneous contractions of ileum fragment by aqueous extract ranging from 24.48 to 90.19 %, for 2.91, to 15.25 mg/mL, respectively, while ethanolic extract was from 42.00 to 83.72 %, for 0.99, to 5.66 mg/mL, respectively. Concerning potassium chloride-induced contraction, aqueous extract concentrations (from 2.91 to 15.25 mg/mL) inhibited contractions from 31.46 to 67.23 %; the ethanolic extract (0.99 to 5.66 mg/mL) inhibited from 38.25, to 82.60 % and verapamil from 14.31 to 80.70 %, at 0.06 to 0.34 mg/mL. After administration of ethanolic extract, all tested doses resulted to reduction of Salmonella typhi load in stools and blood, with activities being duration and dose dependent. Conclusion: The lethal dose 50 of the Rumex bequaertii ethanol extract is greater than 2000 mg/kg. Aqueous and ethanolic leaves’ extracts of Rumex bequaertii possess ex-vivo antispasmodic and ethanolic leaves extract of Rumex bequaertii possess antimicrobial activity.

Rumex bequaertii Ex-vivo antispasmodic antimicrobial wistar rats Salmonella typhi

References (20)

1 Effect of prolonged vigabatrin treatment on hematological and biochemical parameters in plasma, liver and kidney of Swiss albino mice [DOI]
2 Herbal medicine in Jordan with special emphasis on less commonly used medicinal herbs [DOI]
3 Antiviral activity of Rwandan medicinal plants against human immunodeficiency virus type-1 (HIV-1) [DOI]
4 Rickettsia and Bartonella species in fleas from Reunion Island [DOI]
5 Acute and sub-chronic toxicity of a lyophilised aqueous extract of Centaurium erythraea in rodents [DOI]
6 Mechanisms underlying the antihypertensive properties of Urtica dioica [DOI]
7 Nutritional and elemental analyses of some selected medicinal plants of the family Polygonaceae
8 Viral and Bacterial Etiology of Acute Diarrhea among Children under 5 Years of Age in Wuhan, China [DOI]
9 Estimation of Total Phenolic and Total Flavonoid Content and Assessment of in vitro Antioxidant Activity of Extracts of Hamelia patens Jacq. Stems [DOI]
10 Diversity of plants used to treat respiratory diseases in Tubah, northwest region, Cameroon [DOI]
11 Intestinal and vascular smooth muscle relaxant effect of Viscum album explains its medicinal use in hyperactive gut disorders and hypertension [DOI]
12 Immunosuppressive effect of medicinal plants of Kolli hills on mitogen-stimulated proliferation of the human peripheral blood mononuclear cells in vitro [DOI]
13 Pharmacological evaluation of smooth muscle relaxant and cardiac-modulation potential of Phyla nodiflora in ex-vivo and in-vivo experiments [DOI]
14 Muscarinic receptors, nitric oxide formation and cyclooxygenase pathway involved in tracheal smooth muscle relaxant effect of hydro-ethanolic extract of Lavandula angustifolia flowers [DOI]
15 Chemical composition and vascular and intestinal smooth muscle relaxant effects of the essential oil fromPsidium guajavafruit [DOI]
16 Differential regulation of nimodipine-sensitive and -insensitive Ca2+ influx by the Na+/Ca2+ exchanger and mitochondria in the rat suprachiasmatic nucleus neurons [DOI]
17 Spasmogenic and spasmolytic activity of rind of Punica granatum Linn [DOI]
18 Ethnopharmacological basis for antispasmodic, antidiarrheal and antiemetic activities of Ceratonia siliqua pods [DOI]
19 Ulcer Protective and Ulcer Healing Activities of Aqueous and Methanolic Extracts of Leaves of Rumex Bequaertii De Wild (Polygonaceae) in Rats [DOI]
20 Anti-spasmodic activity of Costus afer leaves extract on an isolated rabbit jejunum

Cited by 0

No indexed citations yet.

Review: Parkia speciosa as Valuable, Miracle of Nature — shares topic coverage
Urinary Pathogens amongst Parturients at the University of Port Harcourt Teaching Hospital, South-South, Nigeria — shares topic coverage
Antimicrobial Studies on Leaf and Stem Extracts of Solanum erianthum — shares topic coverage
An in-vitro Analysis of the Antioxidant and Antimicrobial Properties of the Methanol Extract of Hypoxis hemerocallideacorm (MEHHC) from Botswana — shares topic coverage
Evaluation of Antimicrobial Potency and Phytochemical Screening of Persea americana Leaf Extracts against Selected Bacterial and Fungal Isolates of Clinical Importance — shares topic coverage

Access this article

View at publisher

Cite this article

Claude Ruphin Mougoue Deumeni, André Perfusion Amang, Céline Nguefeu Nkenfou, Roméo Georges Bonsou Fosin, Augustin Notedji, Roland Tchuenteu Tchuenguem, Gaël Tchokomeni Siwe, Jules-Roger Kuiate, Pierre Watcho, Paul Vernyuy Tan (2022). Ex-vivo Antispasmodic and In vivo Anti Salmonella Potential of Rumex bequaertii Leaves Extracts. Asian Journal of Research in Biochemistry , 6–14. https://doi.org/10.9734/ajrb/2022/v11i3-4217

Affiliations

Claude Ruphin Mougoue Deumeni — Department of Biological Sciences, Faculty of Science, University of Maroua, P.O. Box 814, Maroua, Cameroon.
André Perfusion Amang — Department of Biological Sciences, Faculty of Science, University of Maroua, P.O. Box 814, Maroua, Cameroon.
Céline Nguefeu Nkenfou — Department of Biological Sciences, Higher Teachers’ Training College, University of Yaoundé I, P.O. Box 47, Yaoundé, Cameroon, Molecular Biology Center, P.O. Box 14495, Yaoundé, Cameroon and Chantal Biya International Reference Centre for Research on HIV/AIDS Prevention and Management, P. O. Box. 3077, Messa, Yaoundé, Cameroon.
Roméo Georges Bonsou Fosin — Department of Animal Biology, Faculty of Sciences, University of Dschang, P.O. Box 67, Dschang, Cameroon.
Augustin Notedji — Molecular Biology Center, P.O. Box 14495, Yaoundé, Cameroon.
Roland Tchuenteu Tchuenguem — Department of Biochemistry, Faculty of Science, University of Dschang, P.O. Box 67 Dschang, Cameroon.
Gaël Tchokomeni Siwe — Department of Animal Biology and Physiology, Faculty of Science, University of Yaoundé I, P.O. Box 812, Yaoundé, Cameroon.
Jules-Roger Kuiate — Department of Biochemistry, Faculty of Science, University of Dschang, P.O. Box 67 Dschang, Cameroon.
Pierre Watcho — Department of Animal Biology, Faculty of Sciences, University of Dschang, P.O. Box 67, Dschang, Cameroon.
Paul Vernyuy Tan — Department of Animal Biology and Physiology, Faculty of Science, University of Yaoundé I, P.O. Box 812, Yaoundé, Cameroon.

Metrics

61
Views: 0
Downloads: 0
Citations: 28
Outbound

Full metrics

Article metrics

Real usage data collected on this platform.

61

Page views

0

PDF downloads

28

Outbound clicks

0

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.