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Research Article Open access CC BY 4.0

Evaluation of Total Phenolic Content and Antioxidant Activity of Three Leaf Extracts of Ziziphus spina-christi (Sedr) Grown in Jordan

Sabah M. J. Khaleel, Adnan S. Jaran, Malik S. Y. Haddadin

Journal of Advances in Medicine and Medical Research · pp. 1–8 · Published 16 Mar 2016

10.9734/BJMMR/2016/24935

Abstract

Aims: The primary aims of this study were to evaluate the total phenolic content and In vitro antioxidant activity of three different leaves extracts (methanolic, ethanolic and aqueous) of Ziziphus spins-christi grown in Jordan. Methods: Total phenolic content of the methanolic, ethanolic and aqueous extracts of the leaves of Ziziphus spins-christi was determined spectrometrically according to the Folin-Ciocalteu procedure. The antioxidant activities of the leaves extracts of this plant at different concentrations were determined by 1,1-diphenyl-2-picryl-hydrazyl (DPPH) scavenging activity and reducing power methods. All the analyzes was made with the use of UV-Visible spectrophotometer, and ascorbic acid was used as a standard antioxidant. Place and Duration of Study: Department of Biological Science, Al-Bayt University, Al Mafraq, Jordan. Department of Nutrition and Food Technology, University of Jordan, Amman, Jordan, from April 2015 to December 2015. Results: The total phenolic content was ranged between 11.8 to 52.5 mg/g expressed in terms of Gallic acid equivalent (mg of GAE/g extract). In vitro antioxidant activity of the plant extracts revealed that all the extracts showed good antioxidant power with IC50 values of 21.4, 24.2 and  54.3 μg/mL for methanolic, aqueous and ethanolic extracts, respectively. The reducing power of the extracts was found to be concentration dependent. The results of this study revealed that, the methanolic extract of leaves showed the highest phenolic concentration and largest antioxidant activity. Conclusion: From this study it may be concluded that Z. spina-christi leaves could have potential source of antioxidants for pharmaceutical drug preparations.

Ziziphus spina-christi total phenolic content DPPH reducing power.

References (34)

  1. 1 [14] Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent [DOI]
  2. 2 Studies on products of browning reaction. Antioxidative activities of products of browning reaction prepared from glucosamine. [DOI]
  3. 3 Free radicals, antioxidants and functional foods: Impact on human health [DOI]
  4. 4 Plant Phenolics: Extraction, Analysis and Their Antioxidant and Anticancer Properties [DOI]
  5. 5 Natural Antioxidants: Sources, Compounds, Mechanisms of Action, and Potential Applications [DOI]
  6. 6 Antioxidant Activity of the Main Phenolic Compounds Isolated from Hot Pepper Fruit (Capsicum annuum L.) [DOI]
  7. 7 Evaluation of solvent effect on the extraction of phenolic compounds and antioxidant capacities from the berries: application of principal component analysis [DOI]
  8. 8 Antioxidant activity of hot water extract from the fruit of the Doum palm, Hyphaene thebaica [DOI]
  9. 9 Ethnopharmacological survey of traditional drugs sold in the Kingdom of Jordan [DOI]
  10. 10 Solvent effects on phytochemical constituent profiles and antioxidant activities, using four different extraction formulations for analysis of Bucida buceras L. and Phoradendron californicum [DOI]
  11. 11 Total phenolic contents and antioxidant activity of corn tassel extracts [DOI]
  12. 12 Extraction of Polyphenolics from Plant Material for Functional Foods—Engineering and Technology [DOI]
  13. 13 Evaluation of antioxidant activity of aqueous extract of some selected nutraceutical herbs [DOI]
  14. 14 Phytochemistry and pharmacologic properties of Ziziphus spina christi (L.) Willd. [DOI]
  15. 15 Essential oil composition and antioxidant and antimicrobial properties of the aerial parts of Salvia eremophila Boiss. from Iran [DOI]
  16. 16 Impact of solvent extraction type on total polyphenols content and biological activity from tamarix aphylla (l.) Karst.
  17. 17 Antioxidant and Free Radical Scavenging Activities of Phellinus merrillii Extracts
  18. 18 Antibacterial and antioxidant activities of ethanol extract from Paullinia cupana Mart. [DOI]
  19. 19 Phytochemical screening, antioxidants and antimicrobial potential of Lantana camara in different solvents [DOI]
  20. 20 Evaluation of the antimicrobial activities of two Ziziphus species (Ziziphus mauritiana L. and Ziziphus spinachristi L.) on some microbial pathogens [DOI]
  21. 21 IN VITRO ANTIOXIDANT ACTIVITY OF METHANOLIC LEAVES AND FLOWERS EXTRACTS OF LIPPIA ALBA
  22. 22 The contribution of fruits and vegetables to dietary intake of polyphenols and antioxidant capacity in a Mexican rural diet: Importance of fruit and vegetable variety [DOI]
  23. 23 Chemical and biological investigations on Zizyphus spina‐christi L. [DOI]
  24. 24 Anticancer Potential of Aqueous Ethanol Seed Extract of Ziziphus mauritiana against Cancer Cell Lines and Ehrlich Ascites Carcinoma [DOI]
  25. 25 Influence of Extraction Solvents on Antioxidant Activity and the Content of Bioactive Compounds in Non-pungent Peppers [DOI]
  26. 26 Studies on in-vitro antioxidant and free radical scavenging potential and phytochemical screening of leaves of Ziziphus mauritiana L.and Ziziphus spina-christi L. compared with Ascorbic acid [DOI]
  27. 27 Antidiabetic and Antioxidant Activities of Zizyphus lotus L Aqueous Extracts in Wistar Rats [DOI]
  28. 28 Assessment of antifungal activity of Rumex vesicarius L. and Ziziphus spina-christi (L.) Willd. extracts against two phytopathogenic fungi [DOI]
  29. 29 Phenolic content and antioxidant properties of green chilli paste and its ingredients
  30. 30 Antibacterial Activity of Francoeuria crispa, Pulicaria undulata, Ziziphus spina-christi and Cucurbita pepo Against Seven Standard Pathogenic Bacteria
  31. 31 Effects of the Zizyphus jujuba Seed Extract on the Lipid Components in Hyperlipidemic Rats [DOI]
  32. 32 Phenolic Compounds of Napek Leave (Zizyphus Spina-Christi L.) as Natural Antioxidants [DOI]
  33. 33 Extraction, Purification and Characterization of Antioxidant Fractions from Zizyphus spina-christi Fruits
  34. 34 The Enhancement of Hawthorn Leaf Extracts on the Growth and Production of Short Chain Fatty Acids of Two Probiotic Bacteria [DOI]

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