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Research Article Open access CC BY 3.0

Evaluation of Polymorphisms rs762624 and rs3176336 of CDKN1A Gene and Risk of Colorectal Cancer

Neda Zali, Mehrdad Hashemi, Vahid Chaleshi, Mahdi Montazer Haghighi, Sanaz Savabkar, Hamid Asadzadeh Aghdaei, Mohsen Vahedi, Ehsan Nazemalhosseini Mojarad, Mohammad Reza Zali

Journal of Advances in Medicine and Medical Research · pp. 5098–5106 · Published 10 Jul 2014

10.9734/BJMMR/2014/8762

Abstract

Aims: Progressive loss of cell cycle control is an important feature on the colorectal cancer. CDKN1A gene encoded p21 protein that’s one of the cyclin-dependent kinase inhibitors, plays a key role in regulating the cell cycle. The aim of this study was to investigate associations of the CDKN1A gene polymorphism rs762624 and rs3176336 with risk of colorectal cancer in an Iranian population. Methods: A case-controls study was conducted to investigate the association of polymorphism rs3176336 and rs762624, with colorectal cancer risk in Iranian population. In this study 150 cases of sporadic CRC and 150 healthy controls were recruited, genomic DNA were extracted from peripheral blood, the genotypes were determined using the polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) method and the result was validated by direct sequencing. Results: The rs762624 frequencies of the AA, AC, and CC genotypes among cases were 9.3%, 74.7%, and 16%, respectively, while in controls genotype frequencies were 10%, 74%, and 16%, respectively. The rs3176336 frequencies of the AA, AC, and CC genotypes among cases were 29.3%, 18% and 52.7%, respectively, while in controls genotype frequencies were18%, 20%, and 62%, respectively. No association was found for the CDKN1A rs3176336 AT/AA genotype (Adjusted odds ratio (OR), 0.726, 95% confidence interval (CI), 0.365–1.443 for AT genotype; OR, 1.67, 95% CI, 0.754–3.702 for AA genotype) with risk of colorectal cancer, compared with the TT genotype. In our research, we could not found significant relation between stage of colorectal cancer and genotypes of rs762624 and rs3176336 polymorphisms (p=0.081, p=0.988). Conclusion: Present data do not confirm association of rs3176336 and rs762624 polymorphisms with susceptibility of Iranian to colorectal cancer.

Single nucleotide polymorphism colorectal cancer CDKN1A rs762624 rs3176336

References (32)

  1. 1 A simple salting out procedure for extracting DNA from human nucleated cells [DOI]
  2. 2 WAF1, a potential mediator of p53 tumor suppression [DOI]
  3. 3 p21 is a universal inhibitor of cyclin kinases [DOI]
  4. 4 p21 in cancer: intricate networks and multiple activities [DOI]
  5. 5 Cell Cycle Control and Cancer [DOI]
  6. 6 Colorectal Cancer Epidemiology: Incidence, Mortality, Survival, and Risk Factors [DOI]
  7. 7 Molecular Genetics of Colorectal Cancer [DOI]
  8. 8 The case of the misleading funnel plot [DOI]
  9. 9 Incidence of Hereditary Nonpolyposis Colorectal Cancer and the Feasibility of Molecular Screening for the Disease [DOI]
  10. 10 Microsatellite instability in colorectal cancer [DOI]
  11. 11 Estimation and projection of the national profile of cancer mortality in China: 1991–2005 [DOI]
  12. 12 An overview of colorectal cancer survival rates and prognosis in Asia [DOI]
  13. 13 Tumor suppressor p53: regulation and function [DOI]
  14. 14 Increasing colorectal cancer incidence rates in Japan [DOI]
  15. 15 An overview of the cell cycle arrest protein, p21WAF1 [DOI]
  16. 16 Association Between EGF, TGF‐β1, VEGF Gene Polymorphism and Colorectal Cancer [DOI]
  17. 17 A functional role for Smad7 in sustaining colon cancer cell growth and survival [DOI]
  18. 18 Association of single-nucleotide polymorphisms in the cell cycle genes with breast cancer in the British population [DOI]
  19. 19 Epidemiology of gastroenterologic cancer in Henan Province, China [DOI]
  20. 20 p21/WAF1/Cip1 expression in invasive ductal breast carcinoma: relationship to p53, proliferation rate, and survival at 5 years [DOI]
  21. 21 Candidate Gene Analysis Using Imputed Genotypes: Cell Cycle Single-Nucleotide Polymorphisms and Ovarian Cancer Risk [DOI]
  22. 22 Low penetrance breast cancer predisposition SNPs are site specific [DOI]
  23. 23 Association of selected polymorphisms of CCND1, p21, and caspase8 With colorectal cancer risk [DOI]
  24. 24 Novel molecular screening approaches in colorectal cancer [DOI]
  25. 25 Effects of Common Germ-Line Genetic Variation in Cell Cycle Genes on Ovarian Cancer Survival [DOI]
  26. 26 A regulatory SNP at position −899 in CDKN1A is associated with systemic lupus erythematosus and lupus nephritis [DOI]
  27. 27 EGF +61A>G polymorphism and gastrointestinal cancer risk: A HuGE review and meta-analysis [DOI]
  28. 28 Association between p21 Ser31Arg polymorphism and cancer risk: a meta-analysis [DOI]
  29. 29 Expression of MSP58 in human colorectal cancer and its correlation with prognosis [DOI]
  30. 30 The effect of 5'untranslated region polymorphism in EGF gene, rs4444903, on colorectal cancer.
  31. 31 Association of p21 Ser31Arg and p53 Arg72Pro polymorphisms with lung cancer risk in CAPUA study [DOI]
  32. 32 DNA damage triggers a prolonged p53-dependent G1 arrest and long-term induction of Cip1 in normal human fibroblasts. [DOI]

Cited by 2

The Correlation Between Single Nucleotide Polymorphism Patterns and Colorectal Cancer in the Iranian Population

Mozhdeh Zamani, Seyed Vahid Hosseini, Pooneh Mokarram · Annals of Colorectal Research · 2016

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